Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ILT3 / LILRB4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115044-100
Catalog Number: LS-G115044
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LILRB4 (NM_006847), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD85K | CD85k antigen | ILT-3 | ILT3 | LILRB4 | LIR-5 | LIR5 | HM18
- Origin Species
- Human
- NCBI GenBank
- NM_006847
- SwissProt
- Q8NHJ6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIPTFTALLC LGLSLGPRTD MQAGPLPKPT LWAEPGSVIS WGNSVTIWCQ GTLEAREYRL61DKEESPAPWD RQNPLEPKNK ARFSIPSMTE DYAGRYRCYY RSPVGWSQPS DPLELVMTGA121YSKPTLSALP SPLVTSGKSV TLLCQSRSPM DTFLLIKERA AHPLLHLRSE HGAQQHQAEF181PMSPVTSVHG GTYRCFSSHG FSHYLLSHPS DPLELIVSGS LEGPRPSPTR SVSTAAGPED241QPLMPTGSVP HSGLRRHWEV LIGVLVVSIL LLSLLLFLLL QHWRQGKHRT LAQRQADFQR301PPGAAEPEPK DGGLQRRSSP AADVQGENFC AAVKNTQPED GVEMDTRQSP HDEDPQAVTY361AKVKHSRPRR EMASPPSPLS GEFLDTKDRQ AEEDRQMDTE AAASEAPQDV TYARLHSFTL421RQKATEPPPS QEGASPAEPS VYATLAIHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
448 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.