Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IL8 / Interleukin 8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99083-100
Catalog Number: LS-G99083
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IL8(CXCL8) (NM_000584), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 3-10C | AMCF-I | B-ENAP | C-X-C motif chemokine 8 | GCP-1 | IL-8 | IL8 | Interleukin-8 | K60 | LECT | GCP1 | LUCT | NAF | NAP1 | Neutrophil-activating factor | Interleukin 8 | Protein 3-10C | MDNCF | TSG-1 | CXCL8 | Emoctakin | LYNAP | MONAP | NAP-1 | SCYB8 | T-cell chemotactic factor
- NCBI GenBank
- NM_000584
- SwissProt
- P10145
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 11.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTSKLAVALL AAFLISAALC EGAVLPRSAK ELRCQCIKTY SKPFHPKFIK ELRVIESGPH61CANTEIIVKL SDGRELCLDP KENWVQRVVE KFLKRAENST RTRPLEQKLI SEEDLAANDI121LDYKDDDDKV
130 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.