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IL31RA Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IL31RA Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IL31RA antibodies →
$725.00each
Catalog Number: LS-G107101-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IL31RA (NM_139017), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CRL | CRL3 | Cytokine receptor-like 3 | GLM-R | GPL | IL-31R subunit alpha | IL-31R-alpha | IL-31RA | Interleukin 31 receptor A | GLMR | HGLM-R | IL-31 receptor subunit alpha | PLCA2 | ZcytoR17 | Class I cytokine receptor | gp130-like monocyte receptor | gp130-like receptor | IL31RA | PRO21384
Origin Species
Human
NCBI GenBank
NM_139017
SwissProt
Q8NI17

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MCIRQLKFFT TACVCECPQN ILSPQPSCVN LGMMWTWALW MLPSLCKFSL AALPAKPENI
61SCVYYYRKNL TCTWSPGKET SYTQYTVKRT YAFGEKHDNC TTNSSTSENR ASCSFFLPRI
121TIPDNYTIEV EAENGDGVIK SHMTYWRLEN IAKTEPPKIF RVKPVLGIKR MIQIEWIKPE
181LAPVSSDLKY TLRFRTVNST SWMEVNFAKN RKDKNQTYNL TGLQPFTEYV IALRCAVKES
241KFWSDWSQEK MGMTEEEAPC GLELWRVLKP AEADGRRPVR LLWKKARGAP VLEKTLGYNI
301WYYPESNTNL TETMNTTNQQ LELHLGGESF WVSMISYNSL GKSPVATLRI PAIQEKSFQC
361IEVMQACVAE DQLVVKWQSS ALDVNTWMIE WFPDVDSEPT TLSWESVSQA TNWTIQQDKL
421KPFWCYNISV YPMLHDKVGE PYSIQAYAKE GVPSEGPETK VENIGVKTVT ITWKEIPKSE
481RKGIICNYTI FYQAEGGKGF SKTVNSSILQ YGLESLKRKT SYIVQVMANT SAGGTNGTSI
541NFKTLSFSVF EIILITSLIG GGLLILIILT VAYGLKKPNK LTHLCWPTVP NPAESSIATW
601HGDDFKDKLN LKESDDSVNT EDRILKPCST PSDKLVIDKL VVNFGNVLQE IFTDEARTGQ
661ENNLGGEKNG YVTCPFRPDC PLGKSFEELP VSPEIPPRKS QYLRSRMPEG TRPEAKEQLL
721FSGQSLVPDH LCEEGAPNPY LKNSVTAREF LVSEKLPEHT KGEV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

764 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.