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IL27RA Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IL27RA Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IL27RA antibodies →
$504.00each
Catalog Number: LS-G116371-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TCCR(IL27RA) (NM_004843), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CRL1 | Cytokine receptor-like 1 | IL-27RA | IL27R | IL-27 receptor subunit alpha | IL-27R | IL-27R subunit alpha | TCCR | WSX-1 | WSX1 | Class I cytokine receptor | IL-27R-alpha | IL27RA | Interleukin 27 receptor | alpha | Zcytor1
Origin Species
Human
NCBI GenBank
NM_004843
SwissProt
Q6UWB1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRGGRGAPFW LWPLPKLALL PLLWVLFQRT RPQGSAGPLQ CYGVGPLGDL NCSWEPLGDL
61GAPSELHLQS QKYRSNKTQT VAVAAGRSWV AIPREQLTMS DKLLVWGTKA GQPLWPPVFV
121NLETQMKPNA PRLGPDVDFS EDDPLEATVH WAPPTWPSHK VLICQFHYRR CQEAAWTLLE
181PELKTIPLTP VEIQDLELAT GYKVYGRCRM EKEEDLWGEW SPILSFQTPP SAPKDVWVSG
241NLCGTPGGEE PLLLWKAPGP CVQVSYKVWF WVGGRELSPE GITCCCSLIP SGAEWARVSA
301VNATSWEPLT NLSLVCLDSA SAPRSVAVSS IAGSTELLVT WQPGPGEPLE HVVDWARDGD
361PLEKLNWVRL PPGNLSALLP GNFTVGVPYR ITVTAVSASG LASASSVWGF REELAPLVGP
421TLWRLQDAPP GTPAIAWGEV PRHQLRGHLT HYTLCAQSGT SPSVCMNVSG NTQSVTLPDL
481PWGPCELWVT ASTIAGQGPP GPILRLHLPD NTLRWKVLPG ILFLWGLFLL GCGLSLATSG
541RCYHLRHKVL PRWVWEKVPD PANSSSGQPH MEQVPEAQPL GDLPILEVEE MEPPPVMESS
601QPAQATAPLD SGYEKHFLPT PEELGLLGPP RPQVLA
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

636 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.