
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IL22RA1 / IL22R Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110663-100
Catalog Number: LS-G110663
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IL22RA1 (NM_021258), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CRF2-9 | IL-22RA1 | IL-22 receptor subunit alpha-1 | IL22R | IL22RA1 | IL-22R-alpha-1 | ZcytoR11 | IL22R1 | Interleukin 22 receptor
- Origin Species
- Human
- NCBI GenBank
- NM_021258
- SwissProt
- Q8N6P7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRTLLTILTV GSLAAHAPED PSDLLQHVKF QSSNFENILT WDSGPEGTPD TVYSIEYKTY61GERDWVAKKG CQRITRKSCN LTVETGNLTE LYYARVTAVS AGGRSATKMT DRFSSLQHTT121LKPPDVTCIS KVRSIQMIVH PTPTPIRAGD GHRLTLEDIF HDLFYHLELQ VNRTYQMHLG181GKQREYEFFG LTPDTEFLGT IMICVPTWAK ESAPYMCRVK TLPDRTWTYS FSGAFLFSMG241FLVAVLCYLS YRYVTKPPAP PNSLNVQRVL TFQPLRFIQE HVLIPVFDLS GPSSLAQPVQ301YSQIRVSGPR EPAGAPQRHS LSEITYLGQP DISILQPSNV PPPQILSPLS YAPNAAPEVG361PPSYAPQVTP EAQFPFYAPQ AISKVQPSSY APQATPDSWP PSYGVCMEGS GKDSPTGTLS421SPKHLRPKGQ LQKEPPAGSC MLGGLSLQEV TSLAMEESQE AKSLHQPLGI CTDRTSDPNV481LHSGEEGTPQ YLKGQLPLLS SVQIEGHPMS LPLQPPSRPC SPSDQGPSPW GLLESLVCPK541DEAKSPAPET SDLEQPTELD SLFRGLALTV QWESTRTRPLEQKLISEEDLAANDILDYKDDDDKV
574 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
