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IL1RAPL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IL1RAPL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112447-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IL1RAPL2 (NM_017416), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IL-1R9 | IL-1RAPL-2 | IL1RAPL-2 | Interleukin 1 receptor 9 | IL1RAPL-2-related protein | IL-1-RAPL-2 | IL-1R-9 | IL1R9 | TIGIRR-1 | IL1RAPL2 | Interleukin-1 receptor 9
Origin Species
Human
NCBI GenBank
NM_017416
SwissProt
Q9NP60

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKPPFLLALV VCSVVSTNLK MVSKRNSVDG CIDWSVDLKT YMALAGEPVR VKCALFYSYI
61RTNYSTAQST GLRLMWYKNK GDLEEPIIFS EVRMSKEEDS IWFHSAEAQD SGFYTCVLRN
121STYCMKVSMS LTVAENESGL CYNSRIRYLE KSEVTKRKEI SCPDMDDFKK SDQEPDVVWY
181KECKPKMWRS IIIQKGNALL IQEVQEEDGG NYTCELKYEG KLVRRTTELK VTALLTDKPP
241KPLFPMENQP SVIDVQLGKP LNIPCKAFFG FSGESGPMIY WMKGEKFIEE LAGHIREGEI
301RLLKEHLGEK EVELALIFDS VVEADLANYT CHVENRNGRK HASVLLRKKD LIYKIELAGG
361LGAIFLLLVL LVVIYKCYNI ELMLFYRQHF GADETNDDNK EYDAYLSYTK VDQDTLDCDN
421PEEEQFALEV LPDVLEKHYG YKLFIPERDL IPSGTYMEDL TRYVEQSRRL IIVLTPDYIL
481RRGWSIFELE SRLHNMLVSG EIKVILIECT ELKGKVNCQE VESLKRSIKL LSLIKWKGSK
541SSKLNSKFWK HLVYEMPIKK KEMLPRCHVL DSAEQGLFGE LQPIPSIAMT STSATLVSSQ
601ADLPEFHPSD SMQIRHCCRG YKHEIPATTL PVPSLGNHHT YCNLPLTLLN GQLPLNNTLK
661DTQEFHRNSS LLPLSSKELS FTSDIW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

686 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.