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IL16 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IL16 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IL16 antibodies →
$725.00each
Catalog Number: LS-G100320-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IL16 (NM_004513), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IL-16 | Interleukin 16 | Neuronal interleukin 16 | PrIL-16 | NIL16 | HsT19289 | IL16 | LCF | PRIL16 | Pro-interleukin-16 | Prointerleukin 16
Origin Species
Human
NCBI GenBank
NM_004513
SwissProt
Q14005

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDYSFDTTAE DPWVRISDCI KNLFSPIMSE NHGHMPLQPN ASLNEEEGTQ GHPDGTPPKL
61DTANGTPKVY KSADSSTVKK GPPVAPKPAW FRQSLKGLRN RASDPRGLPD PALSTQPAPA
121SREHLGSHIR ASSSSSSIRQ RISSFETFGS SQLPDKGAQR LSLQPSSGEA AKPLGKHEEG
181RFSGLLGRGA APTLVPQQPE QVLSSGSPAA SEARDPGVSE SPPPGRQPNQ KTLPPGPDPL
241LRLLSTQAEE SQGPVLKMPS QRARSFPLTR SQSCETKLLD EKTSKLYSIS SQVSSAVMKS
301LLCLPSSISC AQTPCIPKEG ASPTSSSNED SAANGSAETS ALDTGFSLNL SELREYTEGL
361TEAKEDDDGD HSSLQSGQSV ISLLSSEELK KLIEEVKVLD EATLKQLDGI HVTILHKEEG
421AGLGFSLAGG ADLENKVITV HRVFPNGLAS QEGTIQKGNE VLSINGKSLK GTTHHDALAI
481LRQAREPRQA VIVTRKLTPE AMPDLNSSTD SAASASAASD VSVESTAEAT VCTVTLEKMS
541AGLGFSLEGG KGSLHGDKPL TINRIFKGAA SEQSETVQPG DEILQLGGTA MQGLTRFEAW
601NIIKALPDGP VTIVIRRKSL QSKETTAAGD S
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

631 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.