Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IKZF2 / HELIOS Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G120087-100
Catalog Number: LS-G120087
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNFN1A2(IKZF2) (NM_001079526), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ANF1A2 | HELIOS | IKZF2 | Zinc finger protein Helios | ZNF1A2 | ZNFN1A2
- Origin Species
- Human
- NCBI GenBank
- NM_016260
- SwissProt
- Q9UKS7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 56.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1METEAIDGYI TCDNELSPER EHSNMAIDLT SSTPNGQHAS PSHMTSTNSV KLEMQSDEEC61DRKPLSREDE IRGHDEGSSL EEPLIESSEV ADNRKVQELQ GEGGIRLPNG ERPFHCNQCG121ASFTQKGNLL RHIKLHSGEK PFKCPFCSYA CRRRDALTGH LRTHSVGKPH KCNYCGRSYK181QRSSLEEHKE RCHNYLQNVS MEAAGQVMSH HVPPMEDCKE QEPIMDNNIS LVPFERPAVI241EKLTGNMGKR KSSTPQKFVG EKLMRFSYPD IHFDMNLTYE KEAELMQSHM MDQAINNAIT301YLGAEALHPL MQHPPSTIAE VAPVISSAYS QVYHPNRIER PISRETADSH ENNMDGPISL361IRPKSRPQER EASPSNSCLD STDSESSHDD HQSYQGHPAL NPKRKQSPAY MKEDVKALDT421TKAPKGSLKD IYKVFNGEGE QIRAFKCEHC RVLFLDHVMY TIHMGCHGYR DPLECNICGY481RSQDRYEFSS HIVRGEHTFHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
500 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.