Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IK3-1 / CABLES1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107351-100
Catalog Number: LS-G107351
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CABLES1 (NM_138375), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CABL1 | CABLES1 | IK3-1 | HsT2563 | CABLES | Interactor with CDK3 1
- Origin Species
- Human
- NCBI GenBank
- AA587396
- SwissProt
- Q8TDN4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLSKRGCHAR IYADFPIRRL ISQRSSLETL EDIEENAPLR RCRTLSGSPR PKNFKKIHFI61KNMRQHDTRN GRIVLISGRR SFCSIFSVLP YRDSTQVGDL KLDGGRQSTG AVSLKEIIGL121EGVELGADGK TVSYTQFLLP TNAFGARRNT IDSTSSFSQF RNLSHRSLSI GRASGTQGSL181DTGSDLGDFM DYDPNLLDDP QWPCGKHKRV LIFPSYMTTV IDYVKPSDLK KDMNETFKEK241FPHIKLTLSK IRSLKREMRK LAQEDCGLEE PTVAMAFVYF EKLALKGKLN KQNRKLCAGA301CVLLAAKIGS DLKKHEVKHL IDKLEEKFRL NRRELIAFEF PVLVALEFAL HLPEHEVMPH361YRRLVQSSSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
368 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.