Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IIP45 / MIIP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101771-100
Catalog Number: LS-G101771
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MIIP (NM_021933), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IIP45 | MIIP | Invasion-inhibitory protein 45 | RP5-1077B9.4 | IGFBP2-binding protein
- Origin Species
- Human
- NCBI GenBank
- NM_021933
- SwissProt
- Q5JXC2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVEAEELAQL RLLNLELLRQ LWVGQDAVRR SVARAASESS LESSSSYNSE TPSTPETSST61SLSTSCPRGR SSVWGPPDAC RGDLRDVARS GVASLPPANC QHQESLGRPR PHSAPSLGTS121SLRDPEPSGR LGDPGPQEAQ TSRSILAQQS KLSKPRVTFS EESAVPERSW RLRPYLGYDW181IAGSLDTSSS ITSQPEAFFS KLQEFRETNK EECICSHPEP QLPGLRESSG SGVEEDHECV241YCYRVNRRLF PVPVDPGTPC RLCRTPRDQQ GPGTLAQPAH VRVSIPLSIL EPPHRYHIHR301RKSFDASDTL ALPRHCLLGW DIFPPKSEKS SAPRNLDLWS SVSAEAQHQK LSGTSSPFHP361ASPMQMLPPT PTWSVPQVPR PHVPRQKPTR TRPLEQKLIS EEDLAANDIL DYKDDDDKV
419 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.