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IGSF9B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IGSF9B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IGSF9B antibodies →
$725.00each
Catalog Number: LS-G113548-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IGSF9B (NM_014987), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001277285
SwissProt
Q9UPX0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
146.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIWYVATFIA SVIGTRGLAA EGAHGLREEP EFVTARAGES VVLRCDVIHP VTGQPPPYVV
61EWFKFGVPIP IFIKFGYYPP HVDPEYAGRA SLHDKASLRL EQVRSEDQGW YECKVLMLDQ
121QYDTFHNGSW VHLTINAPPT FTETPPQYIE AKEGGSITMT CTAFGNPKPI VTWLKEGTLL
181GASGKYQVSD GSLTVTSVSR EDRGAYTCRA YSIQGEAVHT THLLVQGPPF IVSPPENITV
241NISQDALLTC RAEAYPGNLT YTWYWQDENV YFQNDLKLRV RILIDGTLII FRVKPEDSGK
301YTCVPSNSLG RSPSASAYLT VQYPARVLNM PPVIYVPVGI HGYIRCPVDA EPPATVVKWN
361KDGRPLQVEK NLGWTLMEDG SIRIEEATEE ALGTYTCVPY NTLGTMGQSA PARLVLKDPP
421YFTVLPGWEY RQEAGRELLI PCAAAGDPFP VITWRKVGKP SRSKHSALPS GSLQFRALSK
481EDHGEWECVA TNVVTSITAS THLTVIGTSP HAPGSVRVQV SMTTANVSWE PGYDGGYEQT
541FSVWMKRAQF GPHDWLSLPV PPGPSWLLVD TLEPETAYQF SVLAQNKLGT SAFSEVVTVN
601TLAFPITTPE PLVLVTPPRC LIANRTQQGV LLSWLPPANH SFPIDRYIME FRVAERWELL
661DDGIPGTEGE FFAKDLSQDT WYEFRVLAVM QDLISEPSNI AGVSSTDIFP QPDLTEDGLA
721RPVLAGIVAT ICFLAAAILF STLAACFVNK QRKRKLKRKK DPPLSITHCR KSLESPLSSG
781KVSPESIRTL RAPSESSDDQ GQPAAKRMLS PTREKELSLY KKTKRAISSK KYSVAKAEAE
841AEATTPIELI SRGPDGRFVM DPAEMEPSLK SRRIEGFPFA EETDMYPEFR QSDEENEDPL
901VPTSVAALKS QLTPLSSSQE SYLPPPAYSP RFQPRGLEGP GGLEGRLQAT GQARPPAPRP
961FHHGQYYGYL SSSSPGEVEP PPFYVPEVGS PLSSVMSSPP LPTEGPFGHP TIPEENGENA
1021SNSTLPLTQT PTGGRSPEPW GRPEFPFGGL ETPAMMFPHQ LPPCDVPESL QPKAGLPRGL
1081PPTSLQVPAA YPGILSLEAP KGWAGKSPGR GPVPAPPAAK WQDRPMQPLV SQGQLRHTSQ
1141GMGIPVLPYP EPAEPGAHGG PSTFGLDTRW YEPQPRPRPS PRQARRAEPS LHQVVLQPSR
1201LSPLTQSPLS SRTGSPELAA RARPRPGLLQ QAEMSEITLQ PPAAVSFSRK STPSTGSPSQ
1261SSRSGSPSYR PAMGFTTLAT GYPSPPPGPA PAGPGDSLDV FGQTPSPRRT GEELLRPETP
1321PPTLPTSGKL QRDRPAPATS PPERALSKL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1349 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.