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IGSF9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IGSF9 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IGSF9 antibodies →
$725.00each
Catalog Number: LS-G125380-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IGSF9 (NM_001135050), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FP18798 | Nrt1 | IGSF9 | KIAA1355 | Protein turtle homolog A | IGSF9A
Origin Species
Human
NCBI GenBank
AB037776
SwissProt
Q9P2J2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
126.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVWCLGLAVL SLVISQGADG RGKPEVVSVV GRAGESVVLG CDLLPPAGRP PLHVIEWLRF
61GFLLPIFIQF GLYSPRIDPD YVGRVRLQKG ASLQIEGLRV EDQGWYECRV FFLDQHIPED
121DFANGSWVHL TVNSPPQFQE TPPAVLEVQE LEPVTLRCVA RGSPLPHVTW KLRGKDLGQG
181QGQVQVQNGT LRIRRVERGS SGVYTCQASS TEGSATHATQ LLVLGPPVIV VPPKNSTVNA
241SQDVSLACHA EAYPANLTYS WFQDNINVFH ISRLQPRVRI LVDGSLRLLA TQPDDAGCYT
301CVPSNGLLHP PSASAYLTVL YPAQVTAMPP ETPLPIGMPG VIRCPVRANP PLLFVSWTKD
361GKALQLDKFP GWSQGTEGSL IIALGNEDAL GEYSCTPYNS LGTAGPSPVT RVLLKAPPAF
421IERPKEEYFQ EVGRELLIPC SAQGDPPPVV SWTKVGRGLQ GQAQVDSNSS LILRPLTKEA
481HGHWECSASN AVARVATSTN VYVLGTSPHV VTNVSVVALP KGANVSWEPG FDGGYLQRFS
541VWYTPLAKRP DRMHHDWVSL AVPVGAAHLL VPGLQPHTQY QFSVLAQNKL GSGPFSEIVL
601SAPEGLPTTP AAPGLPPTEI PPPLSPPRGL VAVRTPRGVL LHWDPPELVP KRLDGYVLEG
661RQGSQGWEVL DPAVAGTETE LLVPGLIKDV LYEFRLVAFA GSFVSDPSNT ANVSTSGLEV
721YPSRTQLPGL LPQPVLAGVV GGVCFLGVAV LVSILAGCLL NRRRAARRRR KRLRQDPPLI
781FSPTGKSAAP SALGSGSPDS VAKLKLQGSP VPSLRQSLLW GDPAGTPSPH PDPPSSRGPL
841PLEPICRGPD GRFVMGPTVA APQERSGREQ AEPRTPAQRL ARSFDCSSSS PSGAPQPLCI
901EDISPVAPPP AAPPSPLPGP GPLLQYLSLP FFREMNVDGD WPPLEEPSPA APPDYMDTRR
961CPTSSFLRSP ETPPVSPRES LPGAVVGAGA TAEPPYTALA DWTLRERLLP GLLPAAPRGS
1021LTSQSSGRGS ASFLRPPSTA PSAGGSYLSP APGDTSSWAS GPERWPRREH VVTVSKRRNT
1081SVDENYEWDS EFPGDMELLE TLHLGLASSR LRPEAEPELG VKTPEEGCLL NTAHVTGPEA
1141RCAALREEFL AFRRRRDATR ARLPAYRQPV PHPEQATLL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1179 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.