Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IGSF4C / CADM4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102311-100
Catalog Number: LS-G102311
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cell adhesion molecule 4(CADM4) (NM_145296), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CADM4 | Cell adhesion molecule 4 | Nectin-like 4 | Nectin-like protein 4 | Necl-4 | NECL4 | IGSF4C | TSLC1-like 2 | SynCAM4 | TSLC1-like protein 2 | TSLL2
- Origin Species
- Human
- NCBI GenBank
- NM_145296
- SwissProt
- Q8NFZ8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGRARRFQWP LLLLWAAAAG PGAGQEVQTE NVTVAEGGVA EITCRLHQYD GSIVVIQNPA61RQTLFFNGTR ALKDERFQLE EFSPRRVRIR LSDARLEDEG GYFCQLYTED THHQIATLTV121LVAPENPVVE VREQAVEGGE VELSCLVPRS RPAATLRWYR DRKELKGVSS SQENGKVWSV181ASTVRFRVDR KDDGGIIICE AQNQALPSGH SKQTQYVLDV QYSPTARIHA SQAVVREGDT241LVLTCAVTGN PRPNQIRWNR GNESLPERAE AVGETLTLPG LVSADNGTYT CEASNKHGHA301RALYVLVVYD PGAVVEAQTS VPYAIVGGIL ALLVFLIICV LVGMVWCSVR QKGSYLTHEA361SGLDEQGEAR EAFLNGSDGH KRKEEFFITRTRPLEQKLISEEDLAANDILDYKDDDDKV
388 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.