Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IGSF11 / VSIG3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106178-100
Catalog Number: LS-G106178
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IGSF11 (NM_152538), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BT-IgSF | CXADR like 1 | CXADRL1 | Igsf13 | BTIGSF | VSIG3 | Cancer/testis antigen 119 | CT119 | IGSF11
- Origin Species
- Human
- SwissProt
- Q5DX21
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSLVELLLWW NCFSRTGVAA SLEVSESPGS IQVARGQTAV LPCTFTTSAA LINLNVIWMV61TPLSNANQPE QVILYQGGQM FDGAPRFHGR VGFTGTMPAT NVSIFINNTQ LSDTGTYQCL121VNNLPDIGGR NIGVTGLTVL VPPSAPHCQI QGSQDIGSDV ILLCSSEEGI PRPTYLWEKL181DNTLKLPPTA TQDQVQGTVT IRNISALSSG LYQCVASNAI GTSTCLLDLQ VISPQPRNIG241LIAGAIGTGA VIIIFCIALI LGAFFYWRSK NKEEEEEEIP NEIREDDLPP KCSSAKAFHT301EISSSDNNTL TSSNAYNSRY WSNNPKVHRN TESVSHFSDL GQSFSFHSGN ANIPSIYANG361THLVPGQHKT LVVTANRGSS PQVMSRSNGS VSRKPRPPHT HSYTISHATL ERIGAVPVMV421PAQSRAGSLVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
430 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.