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IGFN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IGFN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G104736-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human eEF1A2 binding protein(IGFN1) (NM_178275), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EEF1A2 binding protein | EEF1A2-binding protein 1 | EEF1A2BP1 | IGFN1 | KY-interacting protein 1 | KYIP1
Origin Species
Human
SwissProt
Q86VF2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
93.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGWQPMGENW GCLEEMLNED QSREPPGHLG SRRSGKDGRL DIYGERRDAT RSSTSRYKPG
61TGSFSKDAQG PMGHFSQGLA DMEVQPGEAA TLSCTLTSDL GPGTWFKDGV KLTTQDGVIF
121KQDGLVHSLF ITHVQGTQAG RYTFVAGDQQ SEATLTVQDS PTIAPDVTEK LREPLVVKAG
181KPVIVKIPFQ SHLPIQAAWR KDGAEVVGSS DREAQVDLGD GYTRLCLPSA GRKDCGQYSV
241TLRSEGGSVQ AELTLQVIDK PDPPQGPMEV QDCHRAGVCL RWRPPRDNGG RTVECYVVER
301RQAGRSTWLK VGEAPADSTT FTDAHVEPGR KYTFRVRAVT SEGAGEALES EEILVAPEAL
361PKAPSAPAIL SASSQGITLT WTAPRGPGSA HILGYLIERR KKGSNTWTAV NDQPVPERRW
421TVADVRQGCQ YEFRVTAVAP SGPGEPGPPS DAVFARDPMR PPGLVRNLQV TDRSNTSITL
481SWAGPDTQEG DEAQGYVVEL CSSDSLQWLP CHVGTVPVTT YTAKGLRPGE GYFVRVTAVN
541EGGQSQPSAL DTLVQAMPVT VCPKFLVDSS TKDLLTVKVG DTVRVPVSFE AMPMPEVTWL
601KDGLPLPKRS VTVTKDGLTQ LLIPVAGLSD SGLYTVVLRT LQGKEVAHSF RIRVAACPQA
661PGPIHLQENV PGTVTAEWEP SPDEAQDVPL HYAVFTRSSA HGPWHEAADR IYTNRFTLLG
721ILPGHEYHFR VVAKNELGAS KPSDTSQPWC IPRQRDRFTV KAPCYREPDL SQKPRFLVGL
781RSHLLPQGCE CCMSCAVQGS PRPHVTWFKN DRSLEGNPAV YSTDLLGVCS LTIPSVSPKD
841SGEYKAVAEN TLGQAVSTAT LIVIEPST
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

868 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.