Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IGFLR1 / TMEM149 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109866-100
Catalog Number: LS-G109866
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM149(IGFLR1) (NM_024660), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IGFLR1 | IGF-like family receptor 1 | Transmembrane protein 149 | TMEM149
- Origin Species
- Human
- NCBI GenBank
- NM_024660
- SwissProt
- Q9H665
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGPGRCLLTA LLLLALAPPP EASQYCGRLE YWNPDNKCCS SCLQRFGPPP CPDYEFRENC61GLNDHGDFVT PPFRKCSSGQ CNPDGAELCS PCGGGAVTPT PAAGGGRTPW RCRERPVPAK121GHCPLTPGNP GAPSSQERSS PASSIAWRTP EPVPQQAWPN FLPLVVLVLL LTLAVIAILL181FILLWHLCWP KEKADPYPYP GLVCGVPNTH TPSSSHLSSP GALETGDTWK EASLLPLLSR241ELSSLASQPL SRLLDELEVL EELIVLLDPE PGPGGGMAHG TTRHLAARYG LPAAWSTFAY301SLRPSRSPLR ALIEMVVARE PSASLGQLGT HLAQLGRADA LRVLSKLGSS GVCWATRTRPLEQKLISEEDLAANDILDYKDDDDKV
355 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.