Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IFT74 / CCDC2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119013-100
Catalog Number: LS-G119013
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CMG1(IFT74) (NM_001099222), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CCDC2 | IFT74 | CMG-1 | CMG1
- Origin Species
- Human
- NCBI GenBank
- NM_025103
- SwissProt
- Q96LB3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 69.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASNHKSSAA RPVSRGGVGL TGRPPSGIRP LSGNIRVATA MPPGTARPGS RGCPIGTGGV61LSSQIKVAHR PVTQQGLTGM KTGTKGPQRQ ILDKSYYLGL LRSKISELTT EVNKLQKGIE121MYNQENSVYL SYEKRAETLA VEIKELQGQL ADYNMLVDKL NTNTEMEEVM NDYNMLKAQN181DRETQSLDVI FTERQAKEKQ IRSVEEEIEQ EKQATDDIIK NMSFENQVKY LEMKTTNEKL241LQELDTLQQQ LDSQNMKKES LEAEIAHSQV KQEAVLLHEK LYELESHRDQ MIAEDKSIGS301PMEEREKLLK QIKDDNQEIA SMERQLTDTK EKINQFIEEI RQLDMDLEEH QGEMNQKYKE361LKKREEHMDT FIETFEETKN QELKRKAQIE ANIVALLEHC SRNINRIEQI SSITNQELKM421MQDDLNFKST EVQKSQSTAQ NLTSDIQRLQ LDLQKMELLE SKMTEEQHSL KSKIKQMTTD481LEIYNDLPAL KSSGEEKIKK LHQERMILST HRNAFKKIME KQNIEYEALK TQLQENETHS541QLTNLERKWQ HLEQNNFAMK EFIATKSQES DYQPIKKNVT KQIAEYNKTI VDALHSTSGNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
600 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.