Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IFT57 / HIPPI Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101521-100
Catalog Number: LS-G101521
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HIPPI(IFT57) (NM_018010), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MHS4R2 | HIP1-interacting protein | IFT57 | DERP8 | ESRRBL1 | HIP1 protein interactor | HIPPI
- Origin Species
- Human
- NCBI GenBank
- AF245220
- SwissProt
- Q9NWB7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTAALAVVTT SGLEDGVPRS RGEGTGEVVL ERGPGAAYHM FVVMEDLVEK LKLLRYEEEF61LRKSNLKAPS RHYFALPTNP GEQFYMFCTL AAWLINKAGR PFEQPQEYDD PNATISNILS121ELRSFGRTAD FPPSKLKSGY GEHVCYVLDC FAEEALKYIG FTWKRPIYPV EELEEESVAE181DDAELTLNKV DEEFVEEETD NEENFIDLNV LKAQTYHLDM NETAKQEDIL ESTTDAAEWS241LEVERVLPQL KVTIRTDNKD WRIHVDQMHQ HRSGIESALK ETKGFLDKLH NEITRTLEKI301SSREKYINNQ LENLVQEYRA AQAQLSEAKE RYQQGNGGVT ERTRLLSEVM EELEKVKQEM361EEKGSSMTDG APLVKIKQSL TKLKQETVEM DIRIGIVEHT LLQSKLKEKS NMTRNMHATV421IPEPATGFYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
429 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.