Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IFT122 / WDR10 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108049-100
Catalog Number: LS-G108049
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IFT122 (NM_052990), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CED | IFT122 | SPG | WDR10 | WDR140 | WDR10p | WD repeat domain 10 | CED1
- Origin Species
- Human
- NCBI GenBank
- NM_018262
- SwissProt
- Q9HBG6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 129.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRAVLTWRDK AEHCINDIAF KPDGTQLILA AGSRLLVYDT SDGTLLQPLK GHKDTVYCVA61YAKDGLWSPE QKSVSKHKSS SKIICCSWTN DGQYLALGMF NGIISIRNKN GEEKVKIERP121GGSLSPIWSI CWNPSREERN DILAVADWGQ KVSFYQLSGK QIGKDRALNF DPCCISYFTK181GEYILLGGSD KQVSLFTKDG VRLGTVGEQN SWVWTCQAKP DSNYVVVGCQ DGTISFYQLI241FSTVHGLYKD RYAYRDSMTD VIVQHLITEQ KVRIKCKELV KKIAIYRNRL AIQLPEKILI301YELYSEDLSD MHYRVKEKII KKFECNLLVV CANHIILCQE KRLQCLSFSG VKEREWQMES361LIRYIKVIGG PPGREGLLVG LKNGQILKIF VDNLFAIVLL KQATAVRCLD MSASRKKLAV421VDENDTCLVY DIDTKELLFQ EPNANSVAWN TQCEDMLCFS GGGYLNIKAS TFPVHRQKLQ481GFVVGYNGSK IFCLHVFSIS AVEVPQSAPM YQYLDRKLFK EAYQIACLGV TDTDWRELAM541EALEGLDFET AKKAFIRVQD LRYLELISSI EERKKRGETN NDLFLADVFS YQGKFHEAAK601LYKRSGHENL ALEMYTDLCM FEYAKDFLGS GDPKETKMLI TKQADWARNI KEPKAAVEMY661ISAGEHVKAI EICGDHGWVD MLIDIARKLD KAEREPLLLC ATYLKKLDSP GYAAETYLKM721GDLKSLVQLH VETQRWDEAF ALGEKHPEFK DDIYMPYAQW LAENDRFEEA QKAFHKAGRQ781REAVQVLEQL TNNAVAESRF NDAAYYYWML SMQCLDIAQA DPAQKDTMLG KFYHFQRLAE841LYHGYHAIHR HTEDPFSVHR PETLFNISRF LLHSLPKDTP SGISKVKILF TLAKQSKALG901AYRLARHAYD KLRGLYIPAR FQKSIELGTL TIRAKPFHDS EELVPLCYRC STNNPLLNNL961GNVCINCRQP FIFSASSYDV LHLVEFYLEE GITDEEAISL IDLEVLRPKR DDRQLEIANN1021SSQILRLVET KDSIGDEDPF TAKLSFEQGG SEFVPVVVSR LVLRSMSRRD VLIKRWPPPL1081RWQYFRSLLP DASITMCPSC FQMFHSEDYE LLVLQHGCCP YCRRCKDDPG PTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1131 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.