Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IFRD1 / TIS7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121969-100
Catalog Number: LS-G121969
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IFRD1 (NM_001007245), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IFRD1 | PC4 | TIS7 | Pheochromocytoma cell-4 | TPA induced sequence 7
- Origin Species
- Human
- NCBI GenBank
- NM_001550
- SwissProt
- O00458
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPKNKKRNTP HRGSSAGGGG SGAAAATAAT AGGQHRNVQP FSDEDASIET MSHCSGYSDP61SSFAEDGPEV LDEEGTQEDL EYKLKGLIDL TLDKSAKTRQ AALEGIKNAL ASKMLYEFIL121ERRMTLTDSI ERCLKKGKSD EQRAAAALAS VLCIQLGPGI ESEEILKTLG PILKKIICDG181SASMQARQTC ATCFGVCCFI ATDDITELYS TLECLENIFT KSYLKEKDTT VICSTPNTVL241HISSLLAWTL LLTICPINEV KKKLEMHFHK LPSLLSCDDV NMRIAAGESL ALLFELARGI301ESDFFYEDME SLTQMLRALA TDGNKHRAKV DKRKQRSVFR DVLRAVEERD FPTETIKFGP361ERMYIDCWVK KHTYDTFKEV LGSGMQYHLQ SNEFLRNVFE LGPPVMLDAA TLKTMKISRF421ERHLYNSAAF KARTKARSKC RDKRADVGEF FTRTRPLEQKLISEEDLAANDILDYKDDDDKV
451 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.