Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IFIX / PYHIN1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G103421-100
Catalog Number: LS-G103421
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PYHIN1 (NM_198930), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IFIX | PYHIN1 | RP11-520H16.1
- Origin Species
- Human
- SwissProt
- Q6K0P9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MANNYKKIVL LKGLEVINDY HFRIVKSLLS NDLKLNPKMK EEYDKIQIAD LMEEKFPGDA61GLGKLIEFFK EIPTLGDLAE TLKREKLKVK GIIPSKKTKQ KEVYPATPAC TPSNRLTAKG121AEETLGPQKR KKPSEEETGT KRSKMSKEQT RPSCSAGAST STAMGRSPPP QTSSSAPPNT181SSTESLKPLA NRHATASKNI FREDPIIAMV LNATKVFKYE SSENEQRRMF HATVATQTQF241FHVKVLNINL KRKFIKKRII IISNYSKRNS LLEVNEASSV SEAGPDQTFE VPKDIIRRAK301KIPKINILHK QTSGYIVYGL FMLHTKIVNR KTTIYEIQDK TGSMAVVGKG ECHNIPCEKG361DKLRLFCFRL RKRENMSKLM SEMHSFIQIQ KNTNQRSHDS RSMALPQEQS QHPKPSEAST421TLPESHLKTP QMPPTTPSSS SFTKVTKDKD IKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
452 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.