
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ICAM4 / CD242 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G120573-100
Catalog Number: LS-G120573
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ICAM4 (NM_001039132), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD242 antigen | ICAM4 | LW | LW blood group protein | ICAM-4 | CD242 | Landsteiner-Wiener blood group
- Origin Species
- Human
- NCBI GenBank
- NM_001544
- SwissProt
- Q14773
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 29.65 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGSLFPLSLL FFLAAAYPGV GSALGRRTKR AQSPKGSPLA PSGTSVPFWV RMSPEFVAVQ61PGKSVQLNCS NSCPQPQNSS LRTPLRQGKT LRGPGWVSYQ LLDVRAWSSL AHCLVTCAGK121TRWATSRITA YSVPGGLLGG DPEAWKPGHL FRKPGALHRP GSGQRDLDLR VCCWTPRLLA181ARDLPRAPQS RRPGGPQQLG THYTDARLEP RAHSFGLRFH RCPCRDPPHC GRCVPMQVPS241YEVPGVKGDV LCRLSEKKRN MKQSGEMAIH GGTRTRPLEQ KLISEEDLAA NDILDYKDDD301DKV
303 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
