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IBTK Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

IBTK Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all IBTK antibodies →
$725.00each
Catalog Number: LS-G113165-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IBTK (NM_015525), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BTBD26 | BTKI | BTK-binding protein | KIAA1417 | RP1-93K22.1 | DKFZP564B116 | IBTK
Origin Species
Human
NCBI GenBank
AL050018
SwissProt
Q9P2D0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
150.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSPMPDCTS KCRSLKHALD VLSVVTKGSE NQIKAFLSSH CYNAATIKDV FGRNALHLVS
61SCGKKGVLDW LIQKGVDLLV KDKESGWTAL HRSIFYGHID CVWSLLKHGV SLYIQDKEGL
121SALDLVMKDR PTHVVFKNTD PTDVYTWGDN TNFTLGHGSQ NSKHHPELVD LFSRSGIYIK
181QVVLCKFHSV FLSQKGQVYT CGHGPGGRLG HGDEQTCLVP RLVEGLNGHN CSQVAAAKDH
241TVVLTEDGCV YTFGLNIFHQ LGIIPPPSSC NVPRQIQAKY LKGRTIIGVA AGRFHTVLWT
301REAVYTMGLN GGQLGCLLDP NGEKCVTAPR QVSALHHKDI ALSLVAASDG ATVCVTTRGD
361IYLLADYQCK KMASKQLNLK KVLVSGGHME YKVDPEHLKE NGGQKICILA MDGAGRVFCW
421RSVNSSLKQC RWAYPRQVFI SDIALNRNEI LFVTQDGEGF RGRWFEEKRK SSEKKEILSN
481LHNSSSDVSY VSDINSVYER IRLEKLTFAH RAVSVSTDPS GCNFAILQSD PKTSLYEIPA
541VSSSSFFEEF GKLLREADEM DSIHDVTFQV GNRLFPAHKY ILAVHSDFFQ KLFLSDGNTS
601EFTDIYQKDE DSAGCHLFVV EKVHPDMFEY LLQFIYTDTC DFLTHGFKPR IHLNKNPEEY
661QGTLNSHLNK VNFHEDDNQK SAFEVYKSNQ AQTVSERQKS KPKSCKKGKN IREDDPVRML
721QTVAKKFDFS NLSSRLDGVR FENEKINVIA KNTGNKLKLS QKKCSFLCDV TMKSVDGKEF
781PCHKCVLCAR LEYFHSMLSS SWIEASSCAA LEMPIHSDIL KVILDYLYTD EAVVIKESQN
841VDFICSVLVV ADQLLITRLK EICEVALTEK LTLKNAAMLL EFAAMYSAKQ LKLSCLQFIG
901LNMAALLEAR SLDVLSDGVL KDLSEFYRKM IPAMDRRVIT PYQDGPDISY LEVEDGDIFL
961KEEINMEQNH SETMFKKAKT KAKKKPRKRS DSSGGYNLSD IIQSPSSTGL LKSGKTNSVE
1021SLPELLTSDS EGSYAGVGSP RDLQSPDFTT GFHSDKIEAK VKPYVNGTSP VYSREDLKPW
1081EKSPILKISA PQPIPSNRID TTSSASWVAG SFSPVSPPVV DLRTIMEIEE SRQKCGATPK
1141SHLGKTVSHG VKLSQKQRKM IALTTKENNS GMNSMETVLF TPSKAPKPVN AWASSLHSVS
1201SKSFRDFLLE EKKSVTSHSS GDHVKKVSFK GIENSQAPKI VRCSTHGTPG PEGNHISDLP
1261LLDSPNPWLS SSVTAPSMVA PVTFASIVEE ELQQEAALIR SREKPLALIQ IEEHAIQDLL
1321VFYEAFGNPE EFVIVERTPQ GPLAVPMWNK HGC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1353 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.