Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
IA-6 / INSM2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108704-100
Catalog Number: LS-G108704
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human INSM2 (NM_032594), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IA6 | IA-6 | Insulinoma-associated 2 | MLT1 | Zinc finger protein IA-6 | INSM2
- Origin Species
- Human
- NCBI GenBank
- NM_032594
- SwissProt
- Q96T92
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPRGFLVKRT KRTGGLYRVR LAERVFPLLG PQGAPPFLEE APSASLPGAE RATPPTREEP61GKGLTAEAAR EQSGSPCRAA GVSPGTGGRE GAEWRAGGRE GPGPSPSPSP SPAKPAGAEL121RRAFLERCLS SPVSAESFPG GAAAVAAFSC SVAPAAAPTP GEQFLLPLRA PFPEPALQPD181PAPLSAALQS LKRAAGGERR GKAPTGCASG PAAAGIKKPK AMRKLSFADE VTTSPVLGLK241IKEEEPGAPS RGLGGSRTPL GEFICQLCKE QYADPFALAQ HRCSRIVRVE YRCPECDKVF301SCPANLASHR RWHKPRPAAA NAATVSSADG KPPSSSSSSS RDSGAIASFL AEGKENSRIE361RTADQHPQAR DSSGADQHPD SAPRQGLQVV THPEPPLPQG PYTEGVLGRR VPVPGSTSGG421RGSEIFVCPY CHKKFRRQAY LRKHLSTHEA GSARALAPGF GSERGAPLAF ACPLCGAHFP481TADIREKHRL WHAVREELLL PALAGAPPET SGPSGPSDGS AQQIFSCKHC PSTFFSSPGL541TRHINKCHPS ESRQVLLLQM PLRPGCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
566 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.