Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HYPE / FICD Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114875-100
Catalog Number: LS-G114875
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HYPE(FICD) (NM_007076), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AMPylator FICD | FIC domain containing | FIC domain-containing protein | HIP13 | Huntingtin yeast partner E | UNQ3041 | FICD | HIP-13 | HYPE
- Origin Species
- Human
- NCBI GenBank
- NM_007076
- SwissProt
- Q9BVA6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MMLIPMASVM AVTEPKWVSV WSRFLWVTLL SMVLGSLLAL LLPLGAVEEQ CLAVLKGLYL61LRSKPDRAQH AATKCTSPST ELSITSRGAT LLVAKTKASP AGKLEARAAL NQALEMKRQG121KREKAQKLFM HALKMDPDFV DALTEFGIFS EEDKDIIQAD YLYTRALTIS PYHEKALVNR181DRTLPLVEEI DQRYFSIIDS KVKKVMSIPK GNSALRRVME ETYYHHIYHT VAIEGNTLTL241SEIRHILETR YAVPGKSLEE QNEVIGMHAA MKYINTTLVS RIGSVTISDV LEIHRRVLGY301VDPVEAGRFR TTQVLVGHHI PPHPQDVEKQ MQEFVQWLNS EEAMNLHPVE FAALAHYKLV361YIHPFIDGNG RTSRLLMNLI LMQAGYPPIT IRKEQRSDYY HVLEAANEGD VRPFIRFIAK421CTETTLDTLL FATTEYSVAL PEAQPNHSGF KETLPVKPTR TRPLEQKLIS EEDLAANDIL481DYKDDDDKV
489 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.