Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HYKK / AGPHD1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121505-100
Catalog Number: LS-G121505
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HYKK (NM_001013619), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 5-hydroxylysine kinase | AGPHD1 | LOC123688
- Origin Species
- Human
- SwissProt
- A2RU49
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSGNYQQSE ALSKPTFSEE QASALVESVF GLKVSKVRPL PSYDDQNFHV YVSKTKDGPT61EYVLKISNTK ASKNPDLIEV QNHIIMFLKA AGFPTASVCH TKGDNTASLV SVDSGSEIKS121YLVRLLTYLP GRPIAELPVS PQLLYEIGKL AAKLDKTLQR FHHPKLSSLH RENFIWNLKN181VPLLEKYLYA LGQNRNREIV EHVIHLFKEE VMTKLSHFRE CINHGDLNDH NILIESSKSA241SGNAEYQVSG ILDFGDMSYG YYVFEVAITI MYMMIESKSP IQVGGHVLAG FESITPLTAV301EKGALFLLVC SRFCQSLVMA AYSCQLYPEN KDYLMVTAKT GWKHLQQMFD MGQKAVEEIW361FETAKSYESG ISMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
373 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.