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Human ZNF451 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ZNF451 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ZNF451 antibodies →
$220.00each
Catalog Number: LS-G79953-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ZNF451 (NM_001031623) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
COASTER | DJ417I1.1 | KIAA0576 | Zinc finger protein 451 | ZNF451 | KIAA1702
Origin Species
Human
NCBI GenBank
AB011148
SwissProt
Q9Y4E5

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
121.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGDPGSEIIE SVPPAGPEAS ESTTDENEDD IQFVSEGPLR PVLEYIDLVS SDDEEPSTSY
61TDENIKRKDH IDYQKDKVAL TLARLARHVE VEKQQKEEKN RAFREKIDFQ HAHGLQELEF
121IRGHSDTEAA RLCVDQWLKM PGLKTGTINC GTKSSFRRGG HTWVSGKPIL CPIMHCNKEF
181DNGHLLLGHL KRFDHSPCDP TITLHGPFFS SFACVVCYKK FVTQQQYRDH LFDKEATDDG
241HNNNLLPQII QCFACPNCFL LFSRKEECSK HMSGKNHFHQ SFKLGDNKGI AHPISFPSFA
301KKLLISLCKD VPFQVKCVAC HKTLRSHMEL TAHFRVHCRN AGPVAVAEKS ITQVAEKFIL
361RGYCPDCNQV FVDETSTQNH KQNSGHKVRV INSVEESVLL YCHSSEGNKD PSSDLHLLLD
421QSKFSSLKRT MSIKESSSLE CIAIPKKKMN LKDKSHEGVA CVQKEKSVVK TWFCECNQRF
481PSEDAVEKHV FSANTMGYKC VVCGKVCDDS GVIRLHMSRI HGGAHLNNFL FWCRTCKKEL
541TRKDTIMAHV TEFHNGHRYF YEMDEVEGET LPSSSTTLDN LTANKPSSAI TVIDHSPANS
601SPRGKWQCRI CEDMFDSQEY VKQHCMSLAS HKFHRYSCAH CRKPFHKIET LYRHCQDEHD
661NEIKIKYFCG LCDLIFNVEE AFLSHYEEHH SIDYVFVSEK TETSIKTEDD FPVIETSNQL
721TCGCRESYIC KVNRKEDYSR CLQIMLDKGK LWFRCSLCSA TAQNLTDMNT HIHQVHKEKS
781DEEEQQYVIK CGTCTKAFHD PESAQQHFHR KHCFLQKPSV AHFGSEKSNL YKFTASASHT
841ERKLKQAINY SKSLDMEKGV ENDLSYQNIE EEIVELPDLD YLRTMTHIVF VDFDNWSNFF
901GHLPGHLNQG TFIWGFQGGN TNWKPPLNCK IYNYLNRIGC FFLHPRCSKR KDAADFAICM
961HAGRLDEQLP KQIPFTILSG DQGFLELENQ FKKTQRPAHI LNPHHLEGDM MCALLNSISD
1021TTKECDSDDN MGAKNTSIGE EFISTEDVEL EEAIRRSLEE M
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1061 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.