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Human ZNF395 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ZNF395 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ZNF395 antibodies →
$153.00each
Catalog Number: LS-G91047-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ZNF395(ZNF395) (NM_018660) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HDRF-2 | HD-regulating factor 2 | HDBP-2 | HDBP2 | Papillomavirus-binding factor | PBF | PRF-1 | Zinc finger protein 395 | ZNF395 | Si-1-8-14
Origin Species
Human
NCBI GenBank
NM_018660
SwissProt
Q9H8N7

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASVLSRRLG KRSLLGARVL GPSASEGPSA APPSEPLLEG AAPQPFTTSD DTPCQEQPKE
61VLKAPSTSGL QQVAFQPGQK VYVWYGGQEC TGLVEQHSWM EGQVTVWLLE QKLQVCCRVE
121EVWLAELQGP CPQAPPLEPG AQALAYRPVS RNIDVPKRKS DAVEMDEMMA AMVLTSLSCS
181PVVQSPPGTE ANFSASRAAC DPWKESGDIS DSGSSTTSGH WSGSSGVSTP SPPHPQASPK
241YLGDAFGSPQ TDHGFETDPD PFLLDEPAPR KRKNSVKVMY KCLWPNCGKV LRSIVGIKRH
301VKALHLGDTV DSDQFKREED FYYTEVQLKE ESAAAAAAAA AGTPVPGTPT SEPAPTPSMT
361GLPLSALPPP LHKAQSSGPE HPGPESSLPS GALSKSAPGS FCHIQADHAY QALPSFQIPV
421SPHIYTSVSW AAAPSAACSL SPVRSRSLSF SEPQQPAPAM KSHLIVTSPP RAQSGARKAR
481GEAKKCRKVY GIEHRDQWCT ACRWKKACQR FLD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

513 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.