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Human ZNF133 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ZNF133 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ZNF133 antibodies →
$220.00each
Catalog Number: LS-G92192-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ZNF133 (NM_001083330) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PHZ-66 | Zinc finger protein 133 | Zinc finger protein 150 | ZNF133 | PHZ-13 | ZNF150
Origin Species
Human
NCBI GenBank
NM_003434
SwissProt
P52736

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAFRDVAVDF TQDEWRLLSP AQRTLYREVM LENYSNLVSL GISFSKPELI TQLEQGKETW
61REEKKCSPAT CPDPEPELYL DPFCPPGFSS QKFPMQHVLC NHPPWIFTCL CAEGNIQPGD
121PGPGDQEKQQ QASEGRPWSD QAEGPEGEGA MPLFGRTKKR TLGAFSRPPQ RQPVSSRNGL
181RGVELEASPA QTGNPEETDK LLKRIEVLGF GTVNCGECGL SFSKMTNLLS HQRIHSGEKP
241YVCGVCEKGF SLKKSLARHQ KAHSGEKPIV CRECGRGFNR KSTLIIHERT HSGEKPYMCS
301ECGRGFSQKS NLIIHQRTHS GEKPYVCREC GKGFSQKSAV VRHQRTHLEE KTIVCSDCGL
361GFSDRSNLIS HQRTHSGEKP YACKECGRCF RQRTTLVNHQ RTHSKEKPYV CGVCGHSFSQ
421NSTLISHRRT HTGEKPYVCG VCGRGFSLKS HLNRHQNIHS GEKPIVCKDC GRGFSQQSNL
481IRHQRTHSGE KPMVCGECGR GFSQKSNLVA HQRTHSGERP YVCRECGRGF SHQAGLIRHK
541RKHSREKPYM CRQCGLGFGN KSALITHKRA HSEEKPCVCR ECGQGFLQKS HLTLHQMTHT
601GEKPYVCKTC GRGFSLKSHL SRHRKTTSVH HRLPVQPDPE PCAGQPSDSL YSL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

653 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.