LSBio
Human ZMAT1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ZMAT1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ZMAT1 antibodies →
$220.00each
Catalog Number: LS-G92980-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ZMAT1 (NM_032441) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1789 | Zinc finger | matrin-type 1 | ZMAT1
Origin Species
Human
NCBI GenBank
NM_001011657
SwissProt
Q5H9K5

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MRTYVCHICS IAFTSLDMFR SHMQGSEHQI KESIVINLVK NSRKTQDSYQ NECADYINVQ
61KARGLEAKTC FRKMEESSLE TRRYREVVDS RPRHRMFEQR LPFETFRTYA APYNISQAME
121KQLPHSKKTY DSFQDELEDY IKVQKARGLD PKTCFRKMRE NSVDTHGYRE MVDSGPRSRM
181CEQRFSHEAS QTYQRPYHIS PVESQLPQWL PTHSKRTYDS FQDELEDYIK VQKARGLEPK
241TCFRKIGDSS VETHRNREMV DVRPRHRMLE QKLPCETFQT YSGPYSISQV VENQLPHCLP
301AHDSKQRLDS ISYCQLTRDC FPEKPVPLSL NQQENNSGSY SVESEVYKHL SSENNTADHQ
361AGHKQKHQKR KRHLEEGKER PEKEQSKHKR KKSYEDTDLD KDKSIRQRKR EEDRVKVSSG
421KLKHRKKKKS HDVPSEKEER KHRKEKKKSV EERTEEEMLW DESILGF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

467 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.