LSBio
Human ZFYVE19 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ZFYVE19 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ZFYVE19 antibodies →
$220.00each
Catalog Number: LS-G85965-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ZFYVE19 (NM_001077268) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MPFYVE | ZFYVE19
Origin Species
Human
NCBI GenBank
NM_032850
SwissProt
Q96K21

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
51.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNYDSQQPPL PPLPYAGCRR ASGFPALGRG GTVPVGVWGG AGQGREGRSW GEGPRGPGLG
61RRDLSSADPA VLGATMESRC YGCAVKFTLF KKEYGCKNCG RAFCSGCLSF SAAVPRTGNT
121QQKVCKQCHE VLTRGSSANA SKWSPPQNYK KRVAALEAKQ KPSTSQSQGL TRQDQMIAER
181LARLRQENKP KLVPSQAEIE ARLAALKDER QGSIPSTQEM EARLAALQGR VLPSQTPQPA
241HHTPDTRTQA QQTQDLLTQL AAEVAIDESW KGGGPAASLQ NDLNQGGPGS TNSKRQANWS
301LEEEKSRLLA EAALELREEN TRQERILALA KRLAMLRGQD PERVTLQDYR LPDSDDDEDE
361ETAIQRVLQQ LTEEASLDEA SGFNIPAEQA SRPWTQPRGA EPEAQDVDPR PEAEEEELPW
421CCICNEDATL RCAGCDGDLF CARCFREGHD AFELKEHQTS AYSPPRAGQE H
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

471 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.