LSBio
Human ZDHHC4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Human ZDHHC4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ZDHHC4 antibodies →
$153.00each
Catalog Number: LS-G85680-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ZDHHC4 (NM_001134387) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DHHC-4 | ZDHHC4 | Zinc finger protein 374 | ZNF374
Origin Species
Human
NCBI GenBank
NM_018106
SwissProt
Q9NPG8

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
39.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MDFLVLFLFY LASVLMGLVL ICVCSKTHSL KGLARGGAQI FSCIIPECLQ RAVHGLLHYL
61FHTRNHTFIV LHLVLQGMVY TEYTWEVFGY CQELELSLHY LLLPYLLLGV NLFFFTLTCG
121TNPGIITKAN ELLFLHVYEF DEVMFPKNVR CSTCDLRKPA RSKHCSVCNW CVHRFDHHCV
181WVNNCIGAWN IRYFLIYVLT LTASAATVAI VSTTFLVHLV VMSDLYQETY IDDLGHLHVM
241DTVFLIQYLF LTFPRIVFML GFVVVLSFLL GGYLLFVLYL AATNQTTNEW YRGDWAWCQR
301CPLVAWPPSA EPQVHRNIHS HGLRSNLQEI FLPAFPCHER KKQE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

344 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.