Human ZCCHC18 Protein (Recombinant His, N-Terminal)
Catalog Number: LS-G144625-50
50 µg / $428.00
Expression System Escherichia coli (Prokaryotic)
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ZCCHC18 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G96545-20
Catalog Number: LS-G96545
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ZCCHC18 (NM_001143978) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASITACVGN SRQQNAPLPP WAHSMLRSLG RSLCPLVVKM AERNMKLFSG RVVPAQGKET61FENWLIQVNE VLPDWSMSEE EKLKRLMKTL RGPAREVMRL LQAANPNLSV ADFLRAMKLV121FGESESSVTA HGKFFNTLQA QGEKASLYVI RLEVQLQNAI QAGILAEKDA NQTRLQQLLL181GAELNRDLRF RLKHLLRMYA NKQERLPNFL ELIKMIREEE DWDDAFIKRK RPKRSEPIME241RAASPVAFQG AQPIAISSAD CNCNVIEIDD TLDDSDEDVI LVVSLYPSLT PTGAPPFRGR301ARPLDQVLVI DSPNNSGAQS LSTSGGSGYK NDGPGNIRRA RKRKYTTRCS YCGEEGHSKE361TCDNESNKAQ VFENLIITLQ ELTHTEERSK EVPGEHSDAS EPQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
403 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
