LSBio
Human WRNIP1 / WHIP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human WRNIP1 / WHIP Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all WRNIP1 antibodies →
$153.00each
Catalog Number: LS-G89052-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human WHIP(WRNIP1) (NM_130395) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Putative helicase RUVBL | WHIP | RP11-420G6.2 | ATPase WRNIP1 | BA420G6.2 | WRNIP1
Origin Species
Human
NCBI GenBank
NM_020135
SwissProt
Q96S55

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEVSGPEDDP FLSQLHQVQC PVCQQMMPAA HINSHLDRCL LLHPAGHAEP AAGSHRAGER
61AKGPSPPGAK RRRLSESSAL KQPATPTAAE SSEGEGEEGD DGGETESRES YDAPPTPSGA
121RLIPDFPVAR SSSPGRKGSG KRPAAAAAAG SASPRSWDEA EAQEEEEAVG DGDGDGDADA
181DGEDDPGHWD ADAAEAATAF GASGGGRPHP RALAAEEIRQ MLQGKPLADT MRPDTLQDYF
241GQSKAVGQDT LLRSLLETNE IPSLILWGPP GCGKTTLAHI IASNSKKHSI RFVTLSATNA
301KTNDVRDVIK QAQNEKSFFK RKTILFIDEI HRFNKSQQVN AALLSRCRVI VLEKLPVEAM
361VTILMRAINS LGIHVLDSSR PTDPLSHSSN SSSEPAMFIE DKAVDTLAYL SDGDARAGLN
421GLQLAVLARL SSRKMFCKKS GQSYSPSRVL ITENDVKEGL QRSHILYDRA GEEHYNCISA
481LHKSMRGSDQ NASLYWLARM LEGGEDPLYV ARRLVRFASE DIGLADPSAL TQAVAAYQGC
541HFIGMPECEV LLAQCVVYFA RAPKSIEVYS AYNNVKACLR NHQGPLPPVP LHLRNAPTRL
601MKDLGYGKGY KYNPMYSEPV DQEYLPEELR GVDFFKQRRC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

640 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.