VWA8 / KIAA0564 Protein (Over-Expression Lysate Myc-DDK (Flag))
Catalog Number: LS-G99271-100
100 µg (1 mg/mL) / $504.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human VWA8 / KIAA0564 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G96056-20
Catalog Number: LS-G96056
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human VWA8 (NM_001009814) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_015058
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 116.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQSRLLLLGA PGGHGGPASR RMRLLLRQVV QRRPGGDRQR PEVRLLHAGS GADTGDTVNI61GDVSYKLKIP KNPELVPQNY ISDSLAQSVV QHLRWIMQKD LLGQDVFLIG PPGPLRRSIA121MQYLELTKRE VEYIALSRDT TETDLKQRRE IRAGTAFYID QCAVRAATEG RTLILEGLEK181AERNVLPVLN NLLENREMQL EDGRFLMSAE RYDKLLRDHT KKELDSWKIV RVSENFRVIA241LGLPVPRYSG NPLDPPLRSR FQARDIYYLP FKDQLKLLYS IGANVSAEKV SQLLSFATTL301CSQESSTLGL PDFPLDSLAA AVQILDSFPM MPIKHAIQWL YPYSILLGHE GKMAVEGVLK361RFELQDSGSS LLPKEIVKVE KMMENHVSQA SVTIRIADKE VTIKVPAGTR LLSQPCASDR421FIQTLSHKQL QAEMMQSHMV KDICLIGGKG CGKTVIAKNF ADTLGYNIEP IMLYQDMTAR481DLLQQRYTLP NGDTAWRSSP LVNAALEGKL VLLDGIHRVN AGTLAVLQRL IHDRELSLYD541GSRLLREDRY MRLKEELQLS DEQLQKRSIF PIHPSFRIIA LAEPPVIGST AHQWLGPEFL601TMFFFHYMKP LVKSEEIQVI KEKVPNVPQE ALDKLLSFTH KLRETQDPTA QSLAASLSTR661QLLRISRRLS QYPNENLHSA VTKACLSRFL PSLARSALEK NLADATIEIN TDDNLEPELK721DYKCEVTSGT LRIGAVSAPI YNAHEKMKVP DVLFYDNIQH VIVMEDMLKD FLLGEHLLLV781GNQGVGKNKI VDRFLHLLNR PREYIQLHRD TTVQTLTLQP SVKDGLIVYE DSPLVKAVKL841GHILVVDEAD KAPTNVTCIL KTLVENGEMI LADGRRIVAN SANVNGRENV VVIHPDFRMI901VLANRPGFPF LGNDFFGTLG DIFSCHAVDN PKPHSELEML RQYGPNVPEP ILQKLVAAFG961ELRSLADQGI INYPYSTREV VNIVKHLQKF PTEGLSSVVR NVFDFDSYNN DMREILINTL1021HKYGIPIGAK PTSVQLAKETRTRPLEQKLISEEDLAANDILDYKDDDDKV
1039 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.