VEGFA / VEGF Antibody
Catalog Number: LS-C154097-100
100 µg (1 mg/mL) / $579.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human
Format: Lyophilized
Human Anti-Ranibizumab Antibody antigen capture ELISA for pharmacokinetic assay developmentA microtiter plate was coated overnight with recombinant human VEGF-A at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of ranibizumab. Detection was performed using Human Anti-Ranibizumab Antibody, clone AbD29928 at a concentration of 2 µg/ml and a rat anti-DYKDDDDK-tag antibody in HISPEC Assay Diluent followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Human VEGFA / VEGF Protein (Recombinant)
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| Expression System | Escherichia coli (Prokaryotic) |
| Format: | Lyophilized |
$902.00each
Catalog Number: LS-G136183-0.2
Catalog Number: LS-G136183
For bulk orders or custom formulations:
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- Description
- Recombinant Human VEGF-AVascular endothelial growth factor alpha forms a homodimer and is a potent stimulator of angiogenesis of both normal and cancerous cells. It acts as a regulator of vasculogenesis, angiogenesis and endothelial cell growth. VEGF-A is secreted by many different cell types such as endothelial cells, smooth muscle cells, neutrophils, platelets, macrophages and 60% of all tumours. The sequence of this recombinant protein product is aa 27-141 + CDKPRR but deficient from aa 142-226, and it is closest to isoform 121. Isoform 121 and 111 lack exons 6 and 7, and for this reason neither of these isoforms bind to the extracellular matrix. This product has been demonstrated for use, in a pharmacokinetic (PK) antigen capture ELISA with the monoclonal antibody drugs ranibizumab and bevacizumab using anti-ranibizumab antibodies for detection.
- Category
- Proteins & Peptides > Proteins > Recombinant Proteins
- Alternative Names
- VPF | Vascular permeability factor | VEGF | VEGF-A | MVCD1 | VEGFA
- Origin Species
- Hu
- NCBI GenBank
- NM_003376
- SwissProt
- P15692
Protein
- Expression System
- E.coli
- Molecular Weight
- ~14 kDa
- Protein Type
- Recombinant
- Amino Acid Sequence
1aa 27-141
9 aa
Format
- Purity
- >90%
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- 20 mM Phosphate Buffer, 0.1 M NaCl, 1% Trehalose
- Concentration
- 0.5 mg/mL
- Purity Measurement Method
- Other
Storage & Handling
- Storage Conditions
- Before reconstitution store at -70°C. Following reconstitution store at -20°C. Store undiluted. Do not use frost-free freezers. Avoid freeze-thaw cycles.
