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Human USP31 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human USP31 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all USP31 antibodies →
$220.00each
Catalog Number: LS-G86669-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human USP31 (NM_020718) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Deubiquitinating enzyme 31 | KIAA1203 | Ubiquitin thioesterase 31 | Ubiquitin thiolesterase 31 | Ubiquitin specific protease 31 | USP31
Origin Species
Human
NCBI GenBank
AB033029
SwissProt
Q70CQ4

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
146.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSKVTAPGSG PPAAASGKEK RSFSKRLFRS GRAGGGGAGG PGASGPAAPS SPSSPSSARS
61VGSFMSRVLK TLSTLSHLSS EGAAPDRGGL RSCFPPGPAA APTPPPCPPP PASPAPPACA
121AEPVPGVAGL RNHGNTCFMN ATLQCLSNTE LFAEYLALGQ YRAGRPEPSP DPEQPAGRGA
181QGQGEVTEQL AHLVRALWTL EYTPQHSRDF KTIVSKNALQ YRGNSQHDAQ EFLLWLLDRV
241HEDLNHSVKQ SGQPPLKPPS ETDMMPEGPS FPVCSTFVQE LFQAQYRSSL TCPHCQKQSN
301TFDPFLCISL PIPLPHTRPL YVTVVYQGKC SHCMRIGVAV PLSGTVARLR EAVSMETKIP
361TDQIVLTEMY YDGFHRSFCD TDDLETVHES DCIFAFETPE IFRPEGILSQ RGIHLNNNLN
421HLKFGLDYHR LSSPTQTAAK QGKMDSPTSR AGSDKIVLLV CNRACTGQQG KRFGLPFVLH
481LEKTIAWDLL QKEILEKMKY FLRPTVCIQV CPFSLRVVSV VGITYLLPQE EQPLCHPIVE
541RALKSCGPGG TAHVKLVVEW DKETRDFLFV NTEDEYIPDA ESVRLQRERH HQPQTCTLSQ
601CFQLYTKEER LAPDDAWRCP HCKQLQQGSI TLSLWTLPDV LIIHLKRFRQ EGDRRMKLQN
661MVKFPLTGLD MTPHVVKRSQ SSWSLPSHWS PWRRPYGLGR DPEDYIYDLY AVCNHHGTMQ
721GGHYTAYCKN SVDGLWYCFD DSDVQQLSED EVCTQTAYIL FYQRRTAIPS WSANSSVAGS
781TSSSLCEHWV SRLPGSKPAS VTSAASSRRT SLASLSESVE MTGERSEDDG GFSTRPFVRS
841VQRQSLSSRS SVTSPLAVNE NCMRPSWSLS AKLQMRSNSP SRFSGDSPIH SSASTLEKIG
901EAADDKVSIS CFGSLRNLSS SYQEPSDSHS RREHKAVGRA PLAVMEGVFK DESDTRRLNS
961SVVDTQSKHS AQGDRLPPLS GPFDNNNQIA YVDQSDSVDS SPVKEVKAPS HPGSLAKKPE
1021STTKRSPSSK GTSEPEKSLR KGRPALASQE SSLSSTSPSS PLPVKVSLKP SRSRSKADSS
1081SRGSGRHSSP APAQPKKESS PKSQDSVSSP SPQKQKSASA LTYTASSTSA KKASGPATRS
1141PFPPGKSRTS DHSLSREGSR QSLGSDRASA TSTSKPNSPR VSQARAGEGR GAGKHVRSSS
1201MASLRSPSTS IKSGLKRDSK SEDKGLSFFK SALRQKETRR STDLGKTALL SKKAGGSSVK
1261SVCKNTGDDE AERGHQPPAS QQPNANTTGK EQLVTKDPAS AKHSLLSARK SKSSQLDSGV
1321PSSPGGRQSA EKSSKKLSSS MQTSARPSQK PQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1352 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.