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Human USHBP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human USHBP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all USHBP1 antibodies →
$153.00each
Catalog Number: LS-G82305-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human USHBP1 (NM_031941) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AIE-75-binding protein | AIEBP | AIE-75 binding protein protein | MCC2 | USH1C-binding protein 1 | USHBP1 | MCC-2
Origin Species
Human
NCBI GenBank
NM_031941
SwissProt
Q8N6Y0

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSARATRPRS RRGRHAPPGE LDPVAESSEE VEAASGSSKP SFAPPPVSSG LEQLGPMEEV
61SGQGLGSRTD KKMDGGSGRE LASAPEVPHK PAVEAHQAPE AALQYKETVP PGNGAPDVFQ
121TLQHTLSSLE AAAAAWRHQP PSHSGPMEFE GTSEGGAGSL GKQEGAGSCQ REAARLAERN
181AWLRLALSSR EDELVRTQAS LEAIRAEKET LQKEVQELQD SLLRLEPCPH LSHNQAGGSG
241SGSSSSEADR EPWETQDSFS LAHPLLRRLR SHSSTQILGS LPNQPLSPEM HIMEAQMEQL
301RGSIEKLKCF NRLLSAVLQG YKGRCEGLSM QLGQREAEAT ALHLALQYSE HCEEAYRVLL
361ALREADSGAG DEAPMSDLQA AEKEAWRLLA QEEAAMDAGA QQNPQPSPEG SSVDKPTPQE
421VAFQLRSYVQ RLQERRSLMK ILSEPGPTLA PMPTVPRAEA MVQAILGTQA GPALPRLEKT
481QIQQDLVAAR EALADLMLRL QLVRREKRGL ELREAALRAL GPAHVLLLEQ LRWERAELQA
541GGANSSGGHS SGGGSSGDEE EWYQGLPAVP GGTSGIDGGQ VGRAWDPEKL AQELAASLTR
601TLDLQEQLQS LRRELEQVAQ KGRARRSQSA ELNRDLCKAH SALVLAFRGA HRKQEEQRRK
661LEQQMALMEA QQAEEVAVLE ATARALGKPR PPLPPPQLGD TFL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

703 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.