LSBio
Human UBN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human UBN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all UBN2 antibodies →
$220.00each
Catalog Number: LS-G95374-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human UBN2 (NM_173569) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA2030 | UBN2 | Ubinuclein 2 | Ubinuclein-2
Origin Species
Human
NCBI GenBank
NM_173569
SwissProt
Q6ZU65

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
145.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEPRRVAFI SLSPVRRREA EYPGPEREPE YPREPPRLEP QPYREPARAE PPAPREPAPR
61SDAQPPSREK PLPQREVSRA EPPMSLQREP PRPEPPPPFP PLPLQPPPPR ESASRAEQPP
121RPPRETVRLE LVLKDPTDES CVEFSYPELL LCGEQRKKLI HTEDPFNDEH QERQEVEMLA
181KKFEMKYGGK PRKHRKDRLQ DLIDIGFGYD ETDPFIDNSE AYDELVPASL TTKYGGFYIN
241TGTLQFRQAS DTEEDDITDN QKHKPPKVPK IKEDDIEMKK RKRKEEGEKE KKPRKKVPKQ
301LGVVALNSHK SEKKKKRYKD SLSLAAMIRK FQKEKDALKK ESNPKVPVTL STPSLNKPPC
361AAAALGNDVP DLNLSSGDPD LPIFVSTNEH ELFQEAENAL EMLDDFDFDR LLDAASDGSP
421LSESGGENGT TTQPTYTSQV MPKVVPTLPE GLPVLLEKRI EDLRVAAKLF DEEGRKKFFT
481QDMNNILLDI ELQLQELGPV IRSGVYSHLE AFVPCNKETL VKRLKKLHLN VQDDRLREPL
541QKLKLAVSNV MPEQLFKYQE DCQARSQAKC AKLQTDEERE KNGSEEDDDE KPGKRVIGPR
601KKFHWDDTIR TLLCNLVEIK LGCYELEPNK SQSAEDYLKS FMETEVKPLW PKGWMQARML
661FKESRSVHNH LTSAPAKKKV IPAPKPKVKE VMVKTLPLHS FPTMLKECSP KKDQKTPTSL
721VASVSGPPTS SSTAAIAAAS SSSAPAQETI CLDDSLDEDL SFHSPSLDLV SEALAVINNG
781NKGPPVGSRI SMPTTKPRPG LREEKLASIM SKLPLATPKK LDSTQTTHSS SLIAGHTGPV
841PKKPQDLAHT GISSGLIAGS SIQNPKVSLE PLPARLLQQG LQRSSQIHTS SSSQTHVSSS
901SQAQIAASSH ALGTSEAQDA SSLTQVTKVH QHSAVQQNYV SPLQATISKS QTNPVVKLSN
961NPQLSCSSSL IKTSDKPLMY RLPLSTPSPG NGSQGSHPLV SRTVPSTTTS SNYLAKAMVS
1021QISTQGFKSP FSMAASPKLA ASPKPATSPK PLPSPKPSAS PKPSLSAKPS VSTKLISKSN
1081PTPKPTVSPS SSSPNALVAQ GSHSSTNSPV HKQPSGMNIS RQSPTLNLLP SSRTSGLPPT
1141KNLQAPSKLT NSSSTGTVGK NSLSGIAMNV PASRGSNLNS SGANRTSLSG GTGSGTQGAT
1201KPLSTPHRPS TASGSSVVTA SVQSTAGASL LANASPLTLM TSPLSVTNQN VTPFGMLGGL
1261VPVTMPFQFP LEIFGFGTDT AGVTTTSGST SAAFHHSLTQ NLLKGLQPGG AQHAATLSHS
1321PLPAHLQQAF HDGGQSKGDT KLPRKSQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1347 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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