UBE1L / UBA7 Antibody
Catalog Number: LS-C415983
2 sizes / from $312.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human UBE1L / UBA7 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G79444-20
Catalog Number: LS-G79444
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Ube1L(UBA7) (NM_003335) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- D8 | UBA1B | UBE1L | UBA7 | Ubiquitin-activating enzyme 7 | UBE2 | Ubiquitin-activating enzyme-2
- Origin Species
- Human
- NCBI GenBank
- NM_003335
- SwissProt
- P41226
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 111.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDALDASKLL DEELYSRQLY VLGSPAMQRI QGARVLVSGL QGLGAEVAKN LVLMGVGSLT61LHDPHPTCWS DLAAQFLLSE QDLERSRAEA SQELLAQLNR AVQVVVHTGD ITEDLLLDFQ121VVVLTAAKLE EQLKVGTLCH KHGVCFLAAD TRGLVGQLFC DFGEDFTVQD PTEAEPLTAA181IQHISQGSPG ILTLRKGANT HYFRDGDLVT FSGIEGMVEL NDCDPRSIHV REDGSLEIGD241TTTFSRYLRG GAITEVKRPK TVRHKSLDTA LLQPHVVAQS SQEVHHAHCL HQAFCALHKF301QHLHGRPPQP WDPVDAETVV GLARDLEPLK RTEEEPLEEP LDEALVRTVA LSSAGVLSPM361VAMLGAVAAQ EVLKAISRKF MPLDQWLYFD ALDCLPEDGE LLPSPEDCAL RGSRYDGQIA421VFGAGFQEKL RRQHYLLVGA GAIGCELLKV FALVGLGAGN SGGLTVVDMD HIERSNLSRQ481FLFRSQDVGR PKAEVAAAAA RGLNPDLQVI PLTYPLDPTT EHIYGDNFFS RVDGVAAALD541SFQARRYVAA RCTHYLKPLL EAGTSGTWGS ATVFMPHVTE AYRAPASAAA SEDAPYPVCT601VRYFPSTAEH TLQWARHEFE ELFRLSAETI NHHQQAHTSL ADMDEPQTLT LLKPVLGVLR661VRPQNWQDCV AWALGHWKLC FHYGIKQLLR HFPPNKVLED GTPFWSGPKQ CPQPLEFDTN721QDTHLLYVLA AANLYAQMHG LPGSQDWTAL RELLKLLPQP DPQQMAPIFA SNLELASASA781EFGPEQQKEL NKALEVWSVG PPLKPLMFEK DDDSNFHVDF VVAAASLRCQ NYGIPPVNRA841QSKRIVGQII PAIATTTAAV AGLLGLELYK VVSGPRPRSA FRHSYLHLAE NYLIRYMPFA901PAIQTFHHLK WTSWDRLKVP AGQPERTLES LLAHLQEQHG LRVRILLHGS ALLYAAGWSP961EKQAQHLPLR VTELVQQLTG QAPAPGQRVL VLELSCEGDD EDTAFPPLHY ELTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1012 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
