LSBio
Human UBASH3A / CLIP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human UBASH3A / CLIP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all UBASH3A antibodies →
$153.00each
Catalog Number: LS-G84988-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human UBASH3A (NM_001001895) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
STS2 | TULA-1 | STS-2 | T-cell ubiquitin ligand 1 | TULA | UBASH3A | Cbl-interacting protein 4
Origin Species
Human
NCBI GenBank
NM_018961
SwissProt
P57075

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAGETQLYA KVSNKLKGRS SPSLLEPFLA MGFPVHTALK ALAATGRKTA EEALAWLHDH
61CNDHSLDDPI PQEYALFLCP TGPLLEKLQE FWRESKRQCA KNRAHEVFPH VTLCDFFTCE
121DQKVECLYEA LKRAGDRLLG SFPTAVPLAL HSSISYLGFF VSGSPADVIR EFAMTFATEA
181SLLADCSVKP YTKQLHLTLA HKFYPHHQRT LEQLARAIPL GHSCQWTAAL YSRDMRFVHY
241QTLRALFQYK PQNVDELTLS PGDYIFVDPT QQDEASEGWV IGISQRTGCR GFLPENYTDR
301ASESDTWVKH RMYTFSLATD LNSRKDGEAS SRCSGEFLPQ TARSLSSLQA LQATIARKSV
361LVVRHGERVD QIFGKAWLQQ CSTPDGKYYR PDLNFPCSLP RRSRGIKDFE NDPPLSSCGI
421FQSRIAGDAL LDSGIRISSV FASPALRCVQ TAKLILEELK LEKKIKIRVE PGIFEWTKWE
481AGKTTPTLMS LEELKEANFN IDTDYRPAFP LSALMPAESY QEYMDRCTAS MVQIVNTCPQ
541DTGVILIVSH GSTLDSCTRP LLGLPPRECG DFAQLVRKIP SLGMCFCEEN KEEGKWELVN
601PPVKTLTHGA NAVFNWRNWI SGN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

623 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.