TUBB8 / Tubulin Beta 8 Antibody (aa34-62)
Catalog Number: LS-C160336-400
400 µL / $401.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human TUBB8 / Tubulin Beta 8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G95590-20
Catalog Number: LS-G95590
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TUBB8 (NM_001164154) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Class VIII beta-tubulin | HSA10p15 beta-tubulin 4Q | RP11-631M21.2 | BA631M21.2 | TUBB8 | Tubulin | beta 8 class VIII | Tubulin beta-8 chain
- Origin Species
- Human
- SwissProt
- Q3ZCM7
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDSVRSGPFG QVFRPDNFIF GQCGAGNNWA KGHYTEGAEL MESVMDVVRK EAESCDCLQG61FQLTHSLGGG TGSGMGTLLL SKIREEYPDR IINTFSILPS PKVSDTVVEP YNATLSVHQL121IENADETFCI DNEALYDICS KTLKLPTPTY GDLNHLVSAT MSGVTTCLRF PGQLNADLRK181LAVNMVPFPR LHFFMPGFAP LTSRGSQQYR ALTVAELTQQ MFDAKNMMAA CDPRHGRYLT241AAAIFRGRMP MREVDEQMFN IQDKNSSYFA DWLPNNVKTA VCDIPPRGLK MSATFIGNNT301AIQELFKRVS EQFTAMFRRK AFLHWYTGEG MDEMEFTEAE SNMNDLVSEY QQYQDATAEE361EEDEEYAEEE VATRTRPLEQKLISEEDLAANDILDYKDDDDKV
372 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
