TS / Thymidylate Synthase Antibody
Catalog Number: LS-C497149
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse
Format: Liquid
Human TS / Thymidylate Synthase Protein (Recombinant His)
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| Expression System | Escherichia coli (Prokaryotic) |
| Format: | Liquid |
$260.00each
Catalog Number: LS-G667-20
Catalog Number: LS-G667
For bulk orders or custom formulations:
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- Description
- Thymidylate synthase is an intracellular enzyme critical for de novo synthesis of DNA. This function maintains the dTMP(thymidine-5-prime monophosphate) pool critical for DNA replication and repair. In cancer, expression of this protein is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-Fu). Resistance or lack of response to 5-Fu is attributed to the elevation of thymidylate synthase activity. Recombinant human Thymidylate synthase protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Category
- Proteins & Peptides > Proteins > Recombinant Proteins
- Alternative Names
- HST422 | Thymidylate synthetase | TMS | TS | TYMS | Thymidylate synthase | Tsase
- Origin Species
- Human
- NCBI GenBank
- NM_001071|NP_001062.1
- SwissProt
- P04818
Protein
- Expression System
- Escherichia coli (Prokaryotic)
- Fusion Tag
- Hexahistidine Tag
- Protein Type
- Recombinant
- Amino Acid Sequence
11-313aa|MG SSHHHHHHSS GLVPRGSHMP VAGSELPRRP LPPAAQERDA EPRPPHGELQ61YLGQIQHILR CGVRKDDRTG TGTLSVFGMQ ARYSLRDEFP LLTTKRVFWK GVLEELLWFI121KGSTNAKELS SKGVKIWDAN GSRDFLDSLG FSTREEGDLG PVYGFQWRHF GAEYRDMESD181YSGQGVDQLQ RVIDTIKTNP DDRRIIMCAW NPRDLPLMAL PPCHALCQFY VVNSELSCQL241YQRSGDMGLG VPFNIASYAL LTYMIAHITG LKPGDFIHTL GDAHIYLNHI EPLKIQLQRE301PRPFPKLRIL RKVEKIDDFK AEDFQIEGYN PHPTIKMEMA V
341 aa
Format
- Purity
- >95%
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 20 mM Tris-HCl, pH 8.0, 0.1 M NaCl, 1 mM DTT, 10% Glycerol
- Purity Measurement Method
- SDS-PAGE (Coomassie Stain)
Storage & Handling
- Storage Conditions
- Short term: store at 2°C to 8°C for up to 1 week. Long term: aliquot and store at -20°C to -80°C. Avoid freeze-thaw cycles.
