TRPM5 Antibody
Catalog Number: LS-C747471
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human TRPM5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G91377-20
Catalog Number: LS-G91377
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TRPM5 (NM_014555) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- LTRPC5 | MLSN1 and TRP-related | MTr1 | TRPM5 | LTrpC-5
- Origin Species
- Human
- NCBI GenBank
- NM_014555
- SwissProt
- Q9NZQ8
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 131.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQDVQGPRPG SPGDAEDRRE LGLHRGEVNF GGSGKKRGKF VRVPSGVAPS VLFDLLLAEW61HLPAPNLVVS LVGEEQPFAM KSWLRDVLRK GLVKAAQSTG AWILTSALRV GLARHVGQAV121RDHSLASTST KVRVVAVGMA SLGRVLHRRI LEEAQEDFPV HYPEDDGGSQ GPLCSLDSNL181SHFILVEPGP PGKGDGLTEL RLRLEKHISE QRAGYGGTGS IEIPVLCLLV NGDPNTLERI241SRAVEQAAPW LILAGSGGIA DVLAALVNQP HLLVPKVAEK QFKEKFPSKH FSWEDIVRWT301KLLQNITSHQ HLLTVYDFEQ EGSEELDTVI LKALVKACKS HSQEPQDYLD ELKLAVAWDR361VDIAKSEIFN GDVEWKSCDL EEVMVDALVS NKPEFVRLFV DNGADVADFL TYGRLQELYR421SVSRKSLLFD LLQRKQEEAR LTLAGLGTQQ AREPPAGPPA FSLHEVSRVL KDFLQDACRG481FYQDGRPGDR RRAEKGPAKR PTGQKWLLDL NQKSENPWRD LFLWAVLQNR HEMATYFWAM541GQEGVAAALA ACKILKEMSH LETEAEAARA TREAKYERLA LDLFSECYSN SEARAFALLV601RRNRCWSKTT CLHLATEADA KAFFAHDGVQ AFLTRIWWGD MAAGTPILRL LGAFLCPALV661YTNLITFSEE APLRTGLEDL QDLDSLDTEK SPLYGLQSRV EELVEAPRAQ GDRGPRAVFL721LTRWRKFWGA PVTVFLGNVV MYFAFLFLFT YVLLVDFRPP PQGPSGPEVT LYFWVFTLVL781EEIRQGFFTD EDTHLVKKFT LYVGDNWNKC DMVAIFLFIV GVTCRMLPSA FEAGRTVLAM841DFMVFTLRLI HIFAIHKQLG PKIIVVERMM KDVFFFLFFL SVWLVAYGVT TQALLHPHDG901RLEWIFRRVL YRPYLQIFGQ IPLDEIDEAR VNCSTHPLLL EDSPSCPSLY ANWLVILLLV961TFLLVTNVLL MNLLIAMFSY TFQVVQGNAD MFWKFQRYNL IVEYHERPAL APPFILLSHL1021SLTLRRVFKK EAEHKREHLE RDLPDPLDQK VVTWETVQKE NFLSKMEKRR RDSEGEVLRK1081TAHRVDFIAK YLGGLREQEK RIKCLESQIN YCSVLVSSVA DVLAQGGGPR SSQHCGEGSQ1141LVAADHRGGL DGWEQPGAGQ PPSDTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1165 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
