TRIM45 Antibody
Catalog Number: LS-C406886
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human TRIM45 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G83811-20
Catalog Number: LS-G83811
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TRIM45 (NM_025188) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- RING finger protein 99 | RNF99 | Tripartite motif containing 45 | Tripartite motif-containing 45 | TRIM45
- Origin Species
- Human
- NCBI GenBank
- NM_025188
- SwissProt
- Q9H8W5
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSENRKPLLG FVSKLTSGTA LGNSGKTHCP LCLGLFKAPR LLPCLHTVCT TCLEQLEPFS61VVDIRGGDSD TSSEGSIFQE LKPRSLQSQI GILCPVCDAQ VDLPMGGVKA LTIDHLAVND121VMLESLRGEG QGLVCDLCND REVEKRCQTC KANLCHFCCQ AHRRQKKTTY HTMVDLKDLK181GYSRIGKPIL CPVHPAEELR LFCEFCDRPV CQDCVVGEHR EHPCDFTSNV IHKHGDSVWE241LLKGTQPHVE ALEEALAQIH IINSALQKRV EAVAADVRTF SEGYIKAIEE HRDKLLKQLE301DIRAQKENSL QLQKAQLEQL LADMRTGVEF TEHLLTSGSD LEILITKRVV VERLRKLNKV361QYSTRPGVND KIRFCPQEKA GQCRGYEIYG TINTKEVDPA KCVLQGEDLH RAREKQTASF421TLLCKDAAGE IMGRGGDNVQ VAVVPKDKKD SPVRTMVQDN KDGTYYISYT PKEPGVYTVW481VCIKEQHVQG SPFTVTVRRK HRPHSGVFHC CTFCSSGGQK TARCACGGTM PGGYLGCGHG541HKGHPGHPHW SCCGKFNEKS ECTWTGGQSA PRSLLRTVALTRTRPLEQKLISEEDLAANDILDYKDDDDKV
580 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
