LSBio
Human TNXB / Tenascin XB Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human TNXB / Tenascin XB Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all TNXB antibodies →
$220.00each
Catalog Number: LS-G94418-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TNXB (NM_032470) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EDS3 | Hexabrachion-like protein | Growth-inhibiting protein 45 | Tenascin-XB | TENX | Tenascin XB | TN-X | TNXB1 | TNXBS | XB | TNX | Tenascin XB1 | Tenascin XB2 | Tenascin-X | TNXB | XBS | HXBL | TNXB2
Origin Species
Human
SwissProt
P22105

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRLSWSVAQG PFDSFVVQYE DTNGQPQALL VDGDQSKILI SGLEPSTPYR FLLYGLHEGK
61RLGPLSAEGT TGLAPAGQTS EESRPRLSQL SVTDVTTSSL RLNWEAPPGA FDSFLLRFGV
121PSPSTLEPHP RPLLQRELMV PGTRHSAVLR DLRSGTLYSL TLYGLRGPHK ADSIQGTART
181LSPVLESPRD LQFSEIRETS AKVNWMPPPS RADSFKVSYQ LADGGEPQSV QVDGQARTQK
241LQGLIPGARY EVTVVSVRGF EESEPLTGFL TTVPDGPTQL RALNLTEGFA VLHWKPPQNP
301VDTYDIQVTA PGAPPLQAET PGSAVDYPLH DLVLHTNYTA TVRGLRGPNL TSPASITFTT
361GLEAPRDLEA KEVTPRTALL TWTEPPVRPA GYLLSFHTPG GQTQEILLPG GITSHQLLGL
421FPSTSYNARL QAMWGQSLLP PVSTSFTTGG LRIPFPRDCG EEMQNGAGAS RTSTIFLNGN
481RERPLIVFCD METDGGGWLV FQRRMDGQTD FWRDWEDYAH GFGNISGEFW LGNEALHSLT
541QAGDYSMRVD LRAGDEAVFA QYDSFHVDSA AEYYRLHLEG YHGTAGDSMS YHSGSVFSAR
601DRDPNSLLIS CAVSYRGAWW YRNCHYANLN GLYGSTVDHQ GVSWYHWKGF EFSVPFTEMK
661LRPRNFRSPA GGG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

673 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.