LSBio
Human TNRC9 / TOX3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human TNRC9 / TOX3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all TOX3 antibodies →
$220.00each
Catalog Number: LS-G81844-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TOX3 (NM_001080430) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CAGF9 | TNRC9 | TOX3
Origin Species
Human
NCBI GenBank
U80736
SwissProt
O15405

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDVRFYPAAA GDPASLDFAQ CLGYYGYSKF GNNNNYMNMA EANNAFFAAS EQTFHTPSLG
61DEEFEIPPIT PPPESDPALG MPDVLLPFQA LSDPLPSQGS EFTPQFPPQS LDLPSITISR
121NLVEQDGVLH SSGLHMDQSH TQVSQYRQDP SLIMRSIVHM TDAARSGVMP PAQLTTINQS
181QLSAQLGLNL GGASMPHTSP SPPASKSATP SPSSSINEED ADEANRAIGE KRAAPDSGKK
241PKTPKKKKKK DPNEPQKPVS AYALFFRDTQ AAIKGQNPNA TFGEVSKIVA SMWDSLGEEQ
301KQVYKRKTEA AKKEYLKALA AYRASLVSKA AAESAEAQTI RSVQQTLAST NLTSSLLLNT
361PLSQHGTVSA SPQTLQQSLP RSIAPKPLTM RLPMNQIVTS VTIAANMPSN IGAPLISSMG
421TTMVGSAPST QVSPSVQTQQ HQMQLQQQQQ QQQQQMQQMQ QQQLQQHQMH QQIQQQMQQQ
481HFQHHMQQHL QQQQQHLQQQ INQQQLQQQL QQRLQLQQLQ HMQHQSQPSP RQHSPVASQI
541TSPIPAIGSP QPASQQHQSQ IQSQTQTQVL SQAIPTICES NCLMNPGTY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

589 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.