LSBio
Human TNF Alpha Protein (Recombinant)

Biological Activity TNF-alpha, Human stimulates cytotoxicity of L-929 mouse fibrosarcoma cells. The ED 50 for this effect is typically 11-50 pg/mL.

Human TNF Alpha Protein (Recombinant)

ProteinLSBio Guarantee
Expression SystemEscherichia coli (Prokaryotic)
Format:Lyophilized
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$268.00each
Catalog Number: LS-G27306-10

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Overview

Description
Tumor Necrosis Factor-alpha (TNF-a) is a homotrimer with a subunit molecular mass of 17.3 kDa. Tumor Necrosis Factor-alpha(TNF-a) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases; and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease. Recombinant Human Tumor Necrosis Factor-alpha (TNF-a) produced in E.coli is a single non-glycosylated polypeptide chain containing 157 amino acids. A fully biologically active molecule, rhTNF-a has a molecular mass of 17.3 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Alternative Names
APC1 protein | Cachectin | DIF | TNF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | macrophage-derived | monocyte-derived | TNFSF2
Origin Species
Human
NCBI GenBank
NM_000594|NP_000585.2
SwissProt
P01375

Protein

Expression System
Escherichia coli (Prokaryotic)
Molecular Weight
17.3 kDa (SDS-PAGE)
Protein Type
Recombinant
Amino Acid Sequence
1VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS
61QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL
121GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

157 aa

Format

Purity
>98%
Conjugate/Label
Unconjugated
Endotoxin Level
< 0.2 EU/µg
Format
Lyophilized
Formulation
Lyophilized from PBS
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Stable at -80°c for up to 6 months. Upon reconstitution, store at 4°c for up to 1 week or at -20°c for up to 3 months.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.