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Human TEX14 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human TEX14 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all TEX14 antibodies →
$220.00each
Catalog Number: LS-G85375-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TEX14 (NM_031272) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SGK307 | Sugen kinase 307 | Testis expressed 14 | Testis expressed sequence 14 | TEX14 | Testis-expressed sequence 14 | Cancer/testis antigen 113 | CT113 | Testis-expressed protein 14
Origin Species
Human
NCBI GenBank
NM_031272
SwissProt
Q8IWB6

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
162.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSRAVRLPVP CPVQLGTLRN DSLEAQLHEY VKQGNYVKVK KILKKGIYVD AVNSLGQTAL
61FVAALLGLRK FVDVLVDYGS DPNHRCFDGS TPVHAAAFSG NQWILSKLLD AGGDLRLHDE
121RGQNPKTWAL TAGKERSTQI VEFMQRCASH MQAIIQGFSY DLLKKIDSPQ RLVYSPSWCG
181GLVQGNPNGS PNRLLKAGVI SAQNIYSFGF GKFYLTGATQ MAYLGSLPVI GEKEVIQADD
241EPTFSFFSGP YMVMTNLVWN GSRVTVKELN LPTHPHCSRL RLADLLIAEQ EHSSKLRHPY
301LLQLMAVCLS QDLEKTRLVY ERITIGTLFS VLHERRSQFP VLHMEVIVHL LLQISDALRY
361LHFQGFIHRS LSSYAVHIIS PGEARLTNLE YMLESEDRGV QRDLTRVPLP TQLYNWAAPE
421VILQKAATVK SDIYSFSMIM QEILTDDIPW KGLDGSVVKK AVVSGNYLEA DVRLPKPYYD
481IVKSGIHVKQ KDRTMNLQDI RYILKNDLKD FTGAQRTQPT ESPRVQRYGL HPDVNVYLGL
541TSEHPRETPD MEIIELKEMG SQPHSPRVHS LFTEGTLDPQ APDPCLMARE TQNQDAPCPA
601PFMAEEASSP STGQPSLCSF EINEIYSGCL ILEDDIEEPP GAASSLEADG PNQVDELKSM
661EEELDKMERE ACCFGSEDES SSKAETEYSF DDWDWQNGSL SSLSLPESTR EAKSNLNNMS
721TTEEYLISKC VLDLKIMQTI MHENDDRLRN IEQILDEVEM KQKEQEERMS LWATSREFTN
781AYKLPLAVGP PSLNYIPPVL QLSGGQKPDT SGNYPTLPRF PRMLPTLCDP GKQNTDEQFQ
841CTQGAKDSLE TSRIQNTSSQ GRPRESTAQA KATQFNSALF TLSSHRQGPS ASPSCHWDST
901RMSVEPVSSE IYNAESRNKD DGKVHLKWKM EVKEMAKKAA TGQLTVPPWH PQSSLTLESE
961AENEPDALLQ PPIRSPENTD WQRVIEYHRE NDEPRGNGKF DKTGNNDCDS DQHGRQPRLG
1021SFTSIRHPSP RQKEQPEHSE AFQASSDTLV AVEKSYSTSS PIEEDFEGIQ GAFAQPQVSG
1081EEKFQMRKIL GKNAEILPRS QFQPVRSTED EQEETSKESP KELKEKDISL TDIQDLSSIS
1141YEPDSSFKEA SCKTPKINHA PTSVSTPLSP GSVSSAASQY KDCLESITFQ VKTEFASCWN
1201SQEFIQTLSD DFISVRERAK KLDSLLTSSE TPPSRLTGLK RLSSFIGAGS PSLVKACDSS
1261PPHATQRRSL PKVEAFSQHH IDELPPPSQE LLDDIELLKQ QQGSSTVLHE NTASDGGGTA
1321NDQRHLEEQE TDSKKEDSSM LLSKETEDLG EDTERAHSTL DEDLERWLQP PEESVELQDL
1381PKGSERETNI KDQKVGEEKR KREDSITPER RKSEGVLGTS EEDELKSCFW KRLGWSESSR
1441IIVLDQSDLS D
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1451 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.