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Human SYNRG Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SYNRG Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SYNRG antibodies →
$220.00each
Catalog Number: LS-G89627-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SYNRG (NM_007247) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Synergin gamma | SYNRG | AP1GBP1 | Gamma-synergin | Synergin | gamma | SYNG
Origin Species
Human
NCBI GenBank
NM_007247
SwissProt
Q9UMZ2

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
140.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MALRPGAGSG GGGAAGAGAG SAGGGGFMFP VAGGIRPPQA GLMPMQQQGF PMVSVMQPNM
61QGIMGMNYSS QMSQGPIAMQ AGIPMGPMPA AGMPYLGQAP FLGMRPPGPQ YTPDMQKQFA
121EEQQKRFEQQ QKLLEEERKR RQFEEQKQKL RLLSSVKPKT GEKSRDDALE AIKGNLDGFS
181RDAKMHPTPA SHPKKPGPSL EEKFLVSCDI STSGQEQIKL NTSEVGHKAL GPGSSKKYPS
241LMASNGVAVD GCVSGTTTAE AENTSDQNLS IEESGVGVFP SQDPAQPRMP PWIYNESLVP
301DAYKKILETT MTPTGIDTAK LYPILMSSGL PRETLGQIWA LANRTTPGKL TKEELYTVLA
361MIAVTQRGVP AMSPDALNQF PAAPIPTLSG FSMTLPTPVS QPTVIPSGPA GSMPLSLGQP
421VMGINLVGPV GGAAAQASSG FIPTYPANQV VKPEEDDFQD FQDASKSGSL DDSFSDFQEL
481PASSKTSNSQ HGNSAPSLLM PLPGTKALPS MDKYAVFKGI AADKSSENTV PPGDPGDKYS
541AFRELEQTAE NKPLGESFAE FRSAGTDDGF TDFKTADSVS PLEPPTKDKT FPPSFPSGTI
601QQKQQTQVKN PLNLADLDMF SSVNCSSEKP LSFSAVFSTS KSVSTPQSTG SAATMTALAA
661TKTSSLADDF GEFSLFGEYS GLAPVGEQDD FADFMAFSNS SISSEQKPDD KYDALKEEAS
721PVPLTSNVGS TVKGGQNSTA ASTKYDVFRQ LSLEGSGLGV EDLKDNTPSG KSDDDFADFH
781SSKFSSINSD KSLGEKAVAF RHTKEDSASV KSLDLPSIGG SSVGKEDSED ALSVQFDMKL
841ADVGGDLKHV MSDSSLDLPT VSGQHPPAAD IEDLKYAAFG SYSSNFAVST LTSYDWSDRD
901DATQGRKLSP FVLSAGSGSP SATSILQKKE TSFGSSENIT MTSLSKVTTF VSEDALPETT
961FPALASFKDT IPQTSEQKEY ENRDYKDFTK QDLPTAERSQ EATCPSPASS GASQETPNEC
1021SDDFGEFQSE KPKISKFDFL VATSQSKMKS SEEMIKSELA TFDLSVQGSH KRSLSLGDKE
1081ISRSSPSPAL EQPFRDRSNT LNEKPALPVI RDKYKDLTGE VEENERYAYE WQRCLGSALN
1141VIKKANDTLN GISSSSVCTE VIQSAQGMEY LLGVVEVYRV TKRVELGIKA TAVCSEKLQQ
1201LLKDIDKVWN NLIGFMSLAT LTPDENSLDF SSCMLRPGIK NAQELACGVC LLNVDSRSRK
1261EEKPAEEHPK KAFNSETDSF KLAYGGHQYH ASCANFWINC VEPKPPGLVL PDLL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1314 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.